Dissecting tumor maintenance requirements using bioluminescence imaging of cell proliferation in a mouse glioma model Journal Article
| Authors: | Uhrbom, L.; Nerio, E.; Holland, E. C. |
| Article Title: | Dissecting tumor maintenance requirements using bioluminescence imaging of cell proliferation in a mouse glioma model |
| Abstract: | Bioluminescence imaging has previously been used to monitor the formation of grafted tumors in vivo and measure cell number during tumor progression and response to therapy. The development and optimization of successful cancer therapy strategies may well require detailed and specific assessment of biological processes in response to mechanistic intervention. Here, we use bioluminescence imaging to monitor the cell cycle in a genetically engineered, histologically accurate model of glioma in vivo. In these platelet-derived growth factor (PDGF)-driven oligodendrogliomas, G1 cell-cycle arrest is generated by blockade of either the PDGF receptor or mTOR using small-molecule inhibitors. |
| Keywords: | immunohistochemistry; controlled study; dna-binding proteins; histopathology; nonhuman; pyridines; glioma; cell proliferation; animal cell; mouse; animals; cell cycle proteins; mice; cell cycle; receptor, platelet-derived growth factor alpha; protein kinases; animal experiment; animal model; in vivo study; animalia; mice, transgenic; transcription factors; genetic engineering; luminescent measurements; platelet-derived growth factor; cell count; oligodendroglioma; dna primers; tumor growth; disease models, animal; luciferases; platelet derived endothelial cell growth factor; cell cycle g1 phase; bioluminescence; phthalazines; in situ nick-end labeling; mitosis inhibition; target of rapamycin kinase; promoter regions (genetics); e2f transcription factors; priority journal; article |
| Journal Title: | Nature Medicine |
| Volume: | 10 |
| Issue: | 11 |
| ISSN: | 1078-8956 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2004-11-01 |
| Start Page: | 1257 |
| End Page: | 1260 |
| Language: | English |
| DOI: | 10.1038/nm1120 |
| PROVIDER: | scopus |
| PUBMED: | 15502845 |
| DOI/URL: | |
| Notes: | Nat. Med. -- Cited By (since 1996):92 -- Export Date: 16 June 2014 -- CODEN: NAMEF -- Source: Scopus |
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