Tumor-specific targeting with modified sindbis viral vectors: Evaluation with optical imaging and positron emission tomography in vivo Journal Article

   

Authors:	Stelter, L.; Tseng, J. C.; Torosjan, A.; Levin, B.; Longo, V. A.; Pillarsetty, N.; Zanzonico, P.; Meruelo, D.; Larson, S. M.
Article Title:	Tumor-specific targeting with modified sindbis viral vectors: Evaluation with optical imaging and positron emission tomography in vivo
Abstract:	Purpose: Sindbis virus (SINV) infect tumor cells specifically and systemically throughout the body. Sindbis vectors are capable of expressing high levels of transduced suicide genes and thus efficiently produce enzymes for prodrug conversion in infected tumor cells. The ability to monitor suicide gene expression levels and viral load in patients, after administration of the vectors, would significantly enhance this tumor-specific therapeutic option. Procedures: The tumor specificity of SINV is mediated by the 67-kDa laminin receptor (LR). We probed different cancer cell lines for their LR expression and, to determine the specific role of LR-expression in the infection cycle, used different molecular imaging strategies, such as bioluminescence, fluorescence molecular tomography, and positron emission tomography, to evaluate SINV-mediated infection in vitro and in vivo. Results: All cancer cell lines showed a marked expression of LR. The infection rates of the SINV particles, however, differed significantly among the cell lines. Conclusion: We used novel molecular imaging techniques to visualize vector delivery to different neoplatic cells. SINV infection rates proofed to be not solely dependent on cellular LR expression. Further studies need to evaluate the herein discussed ways of cellular infection and viral replication. © 2012 World Molecular Imaging Society.
Keywords:	molecular imaging; tumor targeting; pet; bioluminescence; sindbis virus; fmt
Journal Title:	Molecular Imaging and Biology
Volume:	15
Issue:	2
ISSN:	1536-1632
Publisher:	Springer
Date Published:	2013-04-01
Start Page:	166
End Page:	174
Language:	English
DOI:	10.1007/s11307-012-0585-8
PROVIDER:	scopus
PUBMED:	22847302
PMCID:	PMC4429791
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84878503656&partnerID=40&md5=a8c042a46b5dad842c1fa42bbd8371ae
Notes:	--- - "Export Date: 1 July 2013" - "CODEN: CPIMF" - "Source: Scopus"

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