Positional gene expression analysis identifies 12q overexpression and amplification in a subset of neuroblastomas Journal Article

  


Authors: Su, W. T.; Alaminos, M.; Mora, J.; Cheung, N. K.; La Quaglia, M. P.; Gerald, W. L.
Article Title: Positional gene expression analysis identifies 12q overexpression and amplification in a subset of neuroblastomas
Abstract: Neuroblastoma is a heterogeneous disease with variable clinical behaviors. Unique molecular features are associated with clinically relevant subgroups. We performed a comprehensive microarray gene expression analysis of 95 neuroblastomas in an effort to define clinically important molecular subtypes. A subset of tumors overexpressed several contiguous genes located at 12q13∼q15 and were studied further. By microarray, 5 of 95 neuroblastomas had overexpression of genes mapped to 12q13.1∼q15, suggesting an amplification event in this region. Positional expression mapping identified the narrowest region of overlap containing 21 genes, with 11 genes overexpressed in all five cases. Fluorescence in situ hybridization demonstrated 3 neuroblastomas with more than a 10-fold increase in 12q gene copies and 9 with 3- to 5-fold increases. Amplification and overexpression of genes at 12q13∼q15 were observed in a small subset of neuroblastomas. Although amplification of 12q has been previously reported in neuroblastoma cell lines, this is the first demonstration in tumor samples, and it defines a distinct subset that has not been described previously. The expressed genes mapped closely to the complex amplicon reported in sarcomas, and they identify critical genes and pathways affected by 12q gene amplification. © 2004 Elsevier Inc. All rights reserved.
Keywords: human tissue; child, preschool; brain neoplasms; genetic analysis; gene amplification; gene expression; gene expression profiling; gene mapping; fluorescence in situ hybridization; neuroblastoma; oligonucleotide array sequence analysis; dna microarray; chromosome 12q; chromosomes, human, pair 12; humans; human; female; priority journal; article
Journal Title: Cancer Genetics and Cytogenetics
Volume: 154
Issue: 2
ISSN: 0165-4608
Publisher: Elsevier Science, Inc.  
Date Published: 2004-10-15
Start Page: 131
End Page: 137
Language: English
DOI: 10.1016/j.cancergencyto.2004.02.009
PROVIDER: scopus
PUBMED: 15474148
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-5144225768&partnerID=40&md5=655931981cb2555fe0eb478415a8575d
Notes: Cancer Genet. Cytogenet. -- Cited By (since 1996):13 -- Export Date: 16 June 2014 -- CODEN: CGCYD -- Source: Scopus
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MSK Authors
  1. Miguel Alaminos-Mingorance
    9 Alaminos-Mingorance
  2. Wendy Tienhui Su
    11 Su
  3. Michael P La Quaglia
    412 La Quaglia
  4. Nai-Kong Cheung
    650 Cheung
  5. Jaime Mora
    25 Mora
  6. William L Gerald
    375 Gerald
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