| Abstract: |
Cell lines derived from tumors contain numerous chromosomal aberrations and are the focus of study in tumor evolution. The ovarian teratocarcinoma cell line PA-1 demonstrates a single chromosomal aberration: a reciprocal t(15;20)(p11.2;q11.2). A complete molecular genetic analysis was undertaken to characterize this cell line. The PA-1 cell line was studied with fluorescence in situ hybridization (FISH), spectral karyotyping (SKY), bacterial artificial chromosome (BAC) microarray, and Western blotting. Amplification of 20q is frequently implicated in both breast and ovarian cancer; this region contains a number of oncogenes including MDM2, ZNF217, and the ovarian tumor marker WFDC2 (alias HE4). FISH revealed gene amplification of AIB1 (now known as NCOA3) but not STK15 (now known as AURKA). Immunoblot analysis demonstrated 3.6-fold overexpression of the AIB1 protein product, but no elevation of the STK15. BAC cancer gene microarray analysis showed gene amplification of ≥1.20 for five oncogenes. The presence of a consistent single change in PA-1, the t(15;20)(p11.2;q11.2), suggests that the aberration is significant with respect to the transformation status of the cell line. This translocation appears to cause overexpression of AIB1 (and perhaps other proteins), which may provide an immortalizing effect on this cell line. © 2005 Elsevier Inc. All rights reserved. |
| Keywords: |
controlled study; protein expression; unclassified drug; human cell; molecular genetics; genetic analysis; ovarian neoplasms; in situ hybridization, fluorescence; breast cancer; gene amplification; cell line; protein; gene frequency; tumor cells, cultured; chromosomes, human, pair 8; tumor marker; bacterial artificial chromosome; chromosomes, artificial, bacterial; oncogenes; cancer genetics; chromosome aberration; oncogene; blotting, western; fluorescence in situ hybridization; statistical significance; microarray analysis; cell transformation; western blotting; ovary carcinoma; immunoblotting; chromosome translocation; translocation, genetic; dna microarray; chromosome aberrations; alias; spectral karyotyping; chromosomes, human, pair 20; chromosome banding; teratocarcinoma; bacampicillin; protein stk 15; protein wfdc 2; steroid receptor coactivator 3
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