Prognostic value of MYCN and ID2 overexpression in neuroblastoma Journal Article
| Authors: | Alaminos, M.; Gerald, W. L.; Cheung, N. K. V. |
| Article Title: | Prognostic value of MYCN and ID2 overexpression in neuroblastoma |
| Abstract: | Background. The molecular mechanisms driven by overexpression of the MYCN gene in neuroblastoma are not well known. Whether MYCN overexpression in the absence of genomic amplification, or ID2 overexpression has prognostic value remains controversial. Procedure. Ninety-nine neuroblastic tumors from Memorial Sloan-Kettering Cancer Center and 12 neuroblastoma cell lines were analyzed by Affymetrix U95v2 Microarray System. The expression levels of the genes MYCN and ID2 were determined by RT-PCR and immunohistochemistry and the clinical value of the overexpression of both genes was determined. Results. MYCN genomic amplification with overexpression was prognostic for survival (P = 0.0005). Stage 4 patients with MVCN amplification but without overexpression, had no increased likelihood of death, whereas cases with MYCN overexpression but no genomic amplification showed a low survival (P = 0.0096). ID2 did not correlate with MYCN expression (R = -0.23 for tumors and -0.27 for cell lines) or with survival (P = 0.8746). Conclusions. MYCN amplification was not related to clinical outcome in the absence of overexpression in neuroblastoma tumors. ID2 expression appears to be independent of MYCN expression and lacks prognostic value. © 2005 Wiley-Liss, Inc. |
| Keywords: | immunohistochemistry; cancer survival; human tissue; disease-free survival; human cell; cancer staging; gene overexpression; reverse transcription polymerase chain reaction; gene amplification; gene expression profiling; tumor markers, biological; cell line, tumor; cancer mortality; nuclear proteins; oncogene; gene expression regulation, neoplastic; neuroblastoma; neuroblastoma cell; reverse transcriptase polymerase chain reaction; oligonucleotide array sequence analysis; oncogene proteins; device; predictive value of tests; microarray; mycn oncogene; id2 expression; mycn expression; id2 oncogene; inhibitor of differentiation protein 2 |
| Journal Title: | Pediatric Blood and Cancer |
| Volume: | 45 |
| Issue: | 7 |
| ISSN: | 1545-5009 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 2005-12-01 |
| Start Page: | 909 |
| End Page: | 915 |
| Language: | English |
| DOI: | 10.1002/pbc.20505 |
| PUBMED: | 16047351 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 10" - "Export Date: 24 October 2012" - "CODEN: PBCEA" - "Source: Scopus" |
Altmetric
Citation Impact
MSK Authors
Related MSK Work