Monocyte trafficking to hepatic sites of bacterial infection is chemokine independent and directed by focal intercellular adhesion molecule-1 expression Journal Article

   

Authors:	Shi, C.; Velázquez, P.; Hohl, T. M.; Leiner, I.; Dustin, M. L.; Pamer, E. G.
Article Title:	Monocyte trafficking to hepatic sites of bacterial infection is chemokine independent and directed by focal intercellular adhesion molecule-1 expression
Abstract:	Recruitment of CCR2+Ly6Chigh monocytes to sites of infection is essential for efficient clearance of microbial pathogens. Although CCR2-mediated signals promote monocyte emigration from bone marrow, the contribution of CCR2 to later stages of monocyte recruitment remains unresolved. In this article, we show that CCR2 deficiency markedly worsens hepatic Listeria monocytogenes infection because Ly6Chigh monocytes are retained in the bone marrow. Intravenously transferred, CCR2-deficient Ly6Chigh monocytes traffic normally to hepatic foci of infection and contribute to bacterial clearance. Pertussis toxin treatment of adoptively transferred monocytes does not impair their intrahepatic trafficking, suggesting that chemokine signaling, once CCR2+ Ly6Chigh monocytes emigrate from the bone marrow, is not required for monocyte localization to sites of bacterial infection in the liver. Expression of ICAM-1 is induced in close proximity to foci of bacterial infection in the liver, including on CD31+ endothelial cells, and blockade of CD11b and CD44 diminishes monocyte localization to these hepatic foci. Our studies demonstrated that Ly6Chigh monocyte recruitment from the bloodstream to the L. monocytogenes-infected liver does not require chemokine receptor-mediated signals but instead is principally dependent on integrin- and extracellular matrix-mediated monocyte adhesion. Copyright © 2010 by The American Association of Immunologists, Inc.
Keywords:	signal transduction; protein expression; histopathology; pathogenesis; hepatitis; nonhuman; flow cytometry; mouse; animal; cytology; metabolism; animals; mice; animal tissue; bone marrow; animal experiment; cell differentiation; immunofluorescence; inflammatory cell; transgenic mouse; mice, transgenic; endothelium cell; extracellular matrix; fluorescent antibody technique; liver; immunology; disease severity; cd11b antigen; cell migration; cellular distribution; adoptive transfer; monocyte; monocytes; bloodstream infection; microbiology; bacterial infection; cell adhesion; cell separation; intercellular adhesion molecule 1; chemokine receptor ccr2; receptors, ccr2; listeriosis; listeria monocytogenes; neutrophil chemotaxis; chemotaxis, leukocyte; listeria infections; cell selection; cd31 antigen; intercellular adhesion molecule-1; cd44v antigen; pertussis toxin; calcium transport; ccr2 protein, mouse
Journal Title:	Journal of Immunology
Volume:	184
Issue:	11
ISSN:	0022-1767
Publisher:	The American Association of Immunologists, Inc
Date Published:	2010-06-01
Start Page:	6266
End Page:	6274
Language:	English
DOI:	10.4049/jimmunol.0904160
PUBMED:	20435926
PROVIDER:	scopus
PMCID:	PMC2921650
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-77953399156&partnerID=40&md5=2c698a4b5d2f7bda765be3953bdd32a1
Notes:	--- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "CODEN: JOIMA" - "Source: Scopus"

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