B-cell receptors expressed by lymphomas of hepatitis C virus (HCV)-infected patients rarely react with the viral proteins Journal Article
| Authors: | Ng, P. P.; Kuo, C. C.; Wang, S.; Einav, S.; Arcaini, L.; Paulli, M.; Portlock, C. S.; Marcotrigiano, J.; Tarr, A.; Ball, J.; Levy, R.; Levy, S. |
| Article Title: | B-cell receptors expressed by lymphomas of hepatitis C virus (HCV)-infected patients rarely react with the viral proteins |
| Abstract: | Chronic hepatitis C virus (HCV) infection has been implicated in the induction and maintenance of B-cell lymphomas. The strongest evidence for this derives from clinical observations of tumor regressions upon antiviral treatments. Here we used multiple methods to test the hypothesis that the expansion of HCV-specific B cells gives rise to lymphomas. We obtained lymphoma tissues from HCV-infected lymphoma patients, including some that later regressed upon antiviral treatments. We expressed the lymphoma B-cell receptors as soluble immunoglobulin Gs and membrane IgMs, and analyzed their reactivity with HCV proteins and with HCV virions. We confirmed previous reports that HCV-associated lymphomas use a restricted immunoglobulin variable region gene repertoire. However, we found no evidence for their binding to the HCV antigens. We conclude that most lymphomas of HCV-infected patients do not arise from B cells aimed at eliminating the virus. © 2014 by The American Society of Hematology. |
| Keywords: | human tissue; treatment response; major clinical study; hepatitis c; disease association; gene expression; protein binding; immunoreactivity; enzyme linked immunosorbent assay; b lymphocyte; nonhodgkin lymphoma; immunoglobulin variable region; immunoglobulin g; laboratory test; multicenter study; nucleotide sequence; virus antigen; antivirus agent; large cell lymphoma; hepatitis c virus; follicular lymphoma; marginal zone lymphoma; cell expansion; virus protein; immunoglobulin m; antiviral therapy; virion; b lymphocyte receptor; human; priority journal; article |
| Journal Title: | Blood |
| Volume: | 123 |
| Issue: | 10 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2014-03-06 |
| Start Page: | 1512 |
| End Page: | 1515 |
| Language: | English |
| DOI: | 10.1182/blood-2013-10-532895 |
| PROVIDER: | scopus |
| PMCID: | PMC3945861 |
| PUBMED: | 24449209 |
| DOI/URL: | |
| Notes: | Export Date: 1 May 2014 -- CODEN: BLOOA -- Molecular Sequence Numbers: GENBANK: KF895775, KF895776, KF895777, KF895778, KF895779, KF895780, KF895781, KF895782, KF895783, KF895784, KF895785, KF895786, KF895787, KF895788, KF895789, KF895790, KF895791, KF895792, KF895793, KF895794, KF895795, KF895796, KF895797, KF895798, KF895799, KF895800, KF895801, KF895802, KF895803, KF895804, KF895805, KF895806, KF895807, KF895808, KF895809, KF895810, KF895811, KF895812; -- Source: Scopus |
