18F-labeled-bioorthogonal liposomes for in vivo targeting Journal Article
| Authors: | Emmetiere, F.; Irwin, C.; Viola-Villegas, N. T.; Longo, V.; Cheal, S. M.; Zanzonico, P.; Pillarsetty, N.; Weber, W. A.; Lewis, J. S.; Reiner, T. |
| Article Title: | 18F-labeled-bioorthogonal liposomes for in vivo targeting |
| Abstract: | Liposomes are attractive vehicles for the controlled release of drugs and cytotoxins and have a long-standing history in medical research and clinical practice. In addition to established therapeutic indications, liposomes have several favorable properties for molecular imaging, including high stability and the ability to be labeled with radioisotopes, as well as paramagnetic and fluorescent contrast agents. However, long circulation times and difficulties in creating targeted liposomes have proven challenges for imaging. In this study, we have addressed these limitations using a recently developed strategy for bioorthogonal conjugation, the reaction between tetrazines and trans-cyclooctenes. By coating radiolabeled liposomes with trans-cyclooctene and pretargeting with a tetrazine coupled to a targeted peptide, we were able to selectively enhance the retention of liposomes and bind them to tumor tissue in live animals. The rapid reaction between tetrazines and trans-cyclooctenes allowed imaging to be performed with the short-lived PET tracer 18F, yielding signal-to-background activity ratios of 7:1. The covalent, bioorthogonally driven tumor-targeting of liposomes by in vivo click chemistry is promising and should be explored for more selective and rapid delivery of radiodiagnostics and radiotherapeutics, two classes of drugs which particularly benefit from fast clearance, low nonspecific binding, and the associated reduced toxicity to kidneys and bone marrow. © 2013 American Chemical Society. |
| Keywords: | controlled study; unclassified drug; nonhuman; drug targeting; positron emission tomography; mass spectrometry; animal cell; mouse; ovary cancer; animal experiment; animal model; in vivo study; drug distribution; cancer cell; high performance liquid chromatography; drug half life; fluorine 18; liquid chromatography; drug conjugation; liposome; drug degradation; drug conformation; light scattering; electron spin resonance; tetrazine derivative; female; article; alicyclic hydrocarbon; trans cyclooctenes; drug decomposition; gel permeation chromatography |
| Journal Title: | Bioconjugate Chemistry |
| Volume: | 24 |
| Issue: | 11 |
| ISSN: | 1043-1802 |
| Publisher: | American Chemical Society |
| Date Published: | 2013-11-20 |
| Start Page: | 1784 |
| End Page: | 1789 |
| Language: | English |
| DOI: | 10.1021/bc400322h |
| PROVIDER: | scopus |
| PMCID: | PMC3903177 |
| PUBMED: | 24180480 |
| DOI/URL: | |
| Notes: | Export Date: 2 January 2014 -- CODEN: BCCHE -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work