Authors: | Meyer, J. P.; Houghton, J. L.; Kozlowski, P.; Abdel-Atti, D.; Reiner, T.; Pillarsetty, N. V. K.; Scholz, W. W.; Zeglis, B. M.; Lewis, J. S. |
Article Title: | (18)F-based pretargeted PET imaging based on bioorthogonal Diels-Alder click chemistry |
Abstract: | A first-of-its-kind 18F pretargeted PET imaging approach based on the bioorthogonal inverse electron demand Diels-Alder (IEDDA) reaction between tetrazine (Tz) and trans-cyclooctene (TCO) is presented. As proof-of-principle, a TCO-bearing immunoconjugate of the anti-CA19.9 antibody 5B1 and an Al[18F]NOTA-labeled tetrazine radioligand were harnessed for the visualization of CA19.9-expressing BxPC3 pancreatic cancer xenografts. Biodistribution and 18F-PET imaging data clearly demonstrate that this methodology effectively delineates tumor mass with activity concentrations up to 6.4 %ID/g at 4 h after injection of the radioligand. © 2015 American Chemical Society. |
Journal Title: | Bioconjugate Chemistry |
Volume: | 27 |
Issue: | 2 |
ISSN: | 1043-1802 |
Publisher: | American Chemical Society |
Date Published: | 2016-02-17 |
Start Page: | 298 |
End Page: | 301 |
Language: | English |
DOI: | 10.1021/acs.bioconjchem.5b00504 |
PROVIDER: | scopus |
PMCID: | PMC4759614 |
PUBMED: | 26479967 |
DOI/URL: | |
Notes: | Article -- Export Date: 4 April 2016 -- Source: Scopus |