Imaging mouse development with confocal time-lapse microscopy Journal Article
| Authors: | Nowotschin, S.; Ferrer-Vaquer, A.; Hadjantonakis, A. K.; Wassarman, Paul M.; Soriano, Philippe M. |
| Article Title: | Imaging mouse development with confocal time-lapse microscopy |
| Abstract: | The gene expression, signaling, and cellular dynamics driving mouse embryo development have emerged through embryology and genetic studies. However, since mouse development is a temporally regulated three-dimensional process, any insight needs to be placed in this context of real-time visualization. Live imaging using genetically encoded fluorescent protein reporters is pushing the envelope of our understanding by uncovering unprecedented insights into mouse development and leading to the formulation of quantitative accurate models. © 2010 Elsevier Inc. |
| Keywords: | confocal; nonhuman; animals; mice; confocal microscopy; microscopy, confocal; culture medium; fluorescent dyes; developmental biology; time factors; reporter gene; embryo, mammalian; pregnancy; glucose; development; rats; genetic code; sodium chloride; quantum dot; glutamine; magnesium sulfate; confocal laser microscopy; embryonic development; live imaging; embryo culture; culture media; embryo culture techniques; mouse embryo; fluorescent protein; staining and labeling; streptomycin; minimally invasive procedure; illumination; developmental genetics; ex utero culture; reporter strain; time-lapse; cyan fluorescent protein; lactate sodium; phenolsulfonphthalein; potassium chloride; pyruvate sodium; nidation; stereomicroscopy |
| Journal Title: | Methods in Enzymology |
| Volume: | 476 |
| ISSN: | 0076-6879 |
| Publisher: | Academic Press |
| Date Published: | 2010-01-01 |
| Start Page: | 351 |
| End Page: | 377 |
| Language: | English |
| DOI: | 10.1016/s0076-6879(10)76020-1 |
| PUBMED: | 20691876 |
| PROVIDER: | scopus |
| PMCID: | PMC3448272 |
| DOI/URL: | |
| Notes: | - Chapter 20 in "Guide to Techniques in Mouse Development, Part A: Mice, Embryos, and Cells, 2nd Edition" (ISBN: 978-0-12-374775-4) - Cited By (since 1996): 1 - Export Date: 20 April 2011 - CODEN: MENZA - Source: Scopus |
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