Solution structure of a unique G-quadruplex scaffold adopted by a guanosine-rich human intronic sequence Journal Article
| Authors: | Kuryavyi, V.; Patel, D. J. |
| Article Title: | Solution structure of a unique G-quadruplex scaffold adopted by a guanosine-rich human intronic sequence |
| Abstract: | We report on the solution structure of an unprecedented intramolecular G-quadruplex formed by the guanosine-rich human chl1 intronic d(G3-N-G4-N2-G4-N-G3-N) 19-mer sequence in K+-containing solution. This G-quadruplex, composed of three stacked G-tetrads containing four syn guanines, represents a new folding topology with two unique conformational features. The first guanosine is positioned within the central G-tetrad, in contrast to all previous structures of unimolecular G-quadruplexes, where the first guanosine is part of an outermost G-tetrad. In addition, a V-shaped loop, spanning three G-tetrad planes, contains no bridging nucleotides. The G-quadruplex scaffold is stabilized by a T•G•A triple stacked over the G-tetrad at one end and an unpaired guanosine stacked over the G-tetrad at the other end. Finally, the chl1 intronic DNA G-quadruplex scaffold contains a guanosine base intercalated between an extended G-G step, a feature observed in common with the catalytic site of group I introns. This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to intronic G-quadruplex platforms. © 2010 Elsevier Ltd. All rights reserved. |
| Keywords: | controlled study; gene sequence; sequence analysis; protein conformation; protein localization; molecular dynamics; protein stability; intron; introns; nuclear proteins; dna; guanosine; tumor suppressor proteins; base sequence; models, molecular; protein folding; catalytic domain; aqueous solution; g-quadruplexes; guanine quadruplex; enzyme active site; nucleotide; nuclear magnetic resonance, biomolecular; consensus sequence; intercalation complex; scaffold protein |
| Journal Title: | Structure |
| Volume: | 18 |
| Issue: | 1 |
| ISSN: | 0969-2126 |
| Publisher: | Cell Press |
| Date Published: | 2010-01-13 |
| Start Page: | 73 |
| End Page: | 82 |
| Language: | English |
| DOI: | 10.1016/j.str.2009.10.015 |
| PUBMED: | 20152154 |
| PROVIDER: | scopus |
| PMCID: | PMC3381514 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "CODEN: STRUE" - "Source: Scopus" |
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