Selective depletion of eosinophils or neutrophils in mice impacts the efficiency of apoptotic cell clearance in the thymus Journal Article

   

Authors:	Kim, H. J.; Alonzo, E. S.; Dorothee, G.; Pollard, J. W.; Sant'angelo, D. B.
Article Title:	Selective depletion of eosinophils or neutrophils in mice impacts the efficiency of apoptotic cell clearance in the thymus
Abstract:	Developing thymocytes undergo a rigorous selection process to ensure that the mature T cell population expresses a T cell receptor (TCR) repertoire that can functionally interact with major histocompatibility complexes (MHC). Over 90% of thymocytes fail this selection process and die. A small number of macrophages within the thymus are responsible for clearing the large number of dying thymocytes that must be continuously cleared. We studied the capacity of thymic macrophages to clear apoptotic cells under acute circumstances. This was done by synchronously inducing cell death in the thymus and then monitoring the clearance of apoptotic thymocytes. Interestingly, acute cell death was shown to recruit large numbers of CD11b+ cells into the thymus. In the absence of a minor CSF-1 dependent population of macrophages, the recruitment of these CD11b+ cells into the thymus was greatly reduced and the clearance of apoptotic cells was disrupted. To assess a possible role for the CD11b+ cells in the clearance of apoptotic cells, we analyzed mice deficient for eosinophils and mice with defective trafficking of neutrophils. Failure to attract either eosinophils or neutrophils to the thymus resulted in the impaired clearance of apoptotic cells. These results suggested that there is crosstalk between cells of the innate immune system that is necessary for maximizing the efficiency of apoptotic cell removal. © 2010 Kim et al.
Keywords:	immunohistochemistry; controlled study; nonhuman; flow cytometry; antigen expression; t lymphocyte; animal cell; mouse; phenotype; animal; cytology; metabolism; animals; mice; animal tissue; cells, cultured; mus; apoptosis; immune system; cell population; wild type; mice, inbred balb c; mice, inbred c57bl; radiation exposure; physiology; cell type; c57bl mouse; fluorescent antibody technique; immunology; bagg albino mouse; neutrophil; cell culture; thymus; thymus gland; cd11b antigen; cell migration; innate immunity; immunity, innate; neutrophils; bone marrow cell; microbiology; macrophage; macrophages; stroma cell; stromal cells; cell communication; cell selection; colony stimulating factor 1; cd68 antigen; histocompatibility complex; myeloid cells; antigens, cd11b; eosinophil; gamma radiation; eosinophils; gamma rays
Journal Title:	PLoS ONE
Volume:	5
Issue:	7
ISSN:	1932-6203
Publisher:	Public Library of Science
Date Published:	2010-07-06
Start Page:	e11439
Language:	English
DOI:	10.1371/journal.pone.0011439
PUBMED:	20625428
PROVIDER:	scopus
PMCID:	PMC2897847
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-77955360241&partnerID=40&md5=2b3e1bf2fdd2448fde55d937455569fd
Notes:	--- - "Export Date: 20 April 2011" - "Art. No.: e11439" - "Source: Scopus"

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