Immunohistochemical analysis of murine CD95/Fas/Apo-1 receptor and its ultrastructural distribution in the thymus Journal Article


Authors: Ramos, P.; Gangi, M. D.; Baren, A.; Filippa, D.; Elkon, K. B.
Article Title: Immunohistochemical analysis of murine CD95/Fas/Apo-1 receptor and its ultrastructural distribution in the thymus
Abstract: CD95/Fas/Apo-1 is a cell surface receptor that, upon contact with its ligand, induces cells to die by apoptosis. In view of the importance of Fas receptor (FasR) in immunologic tolerance, an immunohistochemical analysis of FasR expression was performed in the lymphoid and certain parenchymal tissues of normal and mutant MRL/lpr mice using a rabbit polyclonal anti-Fas receptor antibody. FasR was expressed by immunoperoxidase (IP) in the cortex and at the corticomedullary junction of the thymus of normal mice. By immunoelectron microscopy FasR was detected on the cell membrane of normal thymocytes. In MRL/lpr mice, FasR protein expression could not be clearly detected. FasR protein expression was not detected in the heart, liver or ovary by IP, presumably reflecting the low number of receptors in these tissues.
Keywords: immunohistochemistry; nonhuman; flow cytometry; polymerase chain reaction; antigen expression; mouse; animals; mice; animal tissue; apoptosis; fas antigen; mice, inbred balb c; immunoenzyme techniques; immune tolerance; tissue distribution; thymus; thymus gland; messenger rna; lymphoid tissue; lymphocytes; cell surface receptor; receptors, cell surface; mice, inbred mrl lpr; immunoelectron microscopy; antigens, cd95; cell ultrastructure; microscopy, immunoelectron; immunoperoxidase staining; male; female; priority journal; article; fasr/cd95; immunoperoxidase
Journal Title: APMIS
Volume: 105
Issue: 7-12
ISSN: 0903-4641
Publisher: Wiley Blackwell  
Date Published: 1997-07-01
Start Page: 597
End Page: 602
Language: English
PUBMED: 9298096
PROVIDER: scopus
DOI: 10.1111/j.1699-0463.1997.tb05058.x
DOI/URL:
Notes: Article -- Export Date: 17 March 2017 -- Source: Scopus
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MSK Authors
  1. Daniel A Filippa
    132 Filippa
  2. Ann Baren
    9 Baren