Age-associated changes in the differentiation potentials of human circulating hematopoietic progenitors to T- or NK-lineage cells Journal Article

   

Authors:	Kyoizumi, S.; Kubo, Y.; Kajimura, J.; Yoshida, K.; Imai, K.; Hayashi, T.; Nakachi, K.; Young, L. F.; Moore, M. A.; van den Brink, M. R. M.; Kusunoki, Y.
Article Title:	Age-associated changes in the differentiation potentials of human circulating hematopoietic progenitors to T- or NK-lineage cells
Abstract:	Age-associated changes of T and NK cell (T/NK) potential of human hematopoietic stem cells are unknown. In this study, we enumerate and characterize T/NK precursors among CD34+Lin- cell populations circulating in normal human adult peripheral blood (PB) by a limiting-dilution assay using coculture with OP9-DL1 stroma cells expressing Notch 1 ligand, Delta-like 1. The frequency of T cell precursors in CD34 +Lin- cells was found to decrease with donor age, whereas the ratio of NK to T cell precursor frequency (NK/T ratio) increased with age, suggesting that lymphoid differentiation potential of PB progenitors shifts from T to NK cell lineage with aging. Clonal analyses of CD34+Lin - cells showed that differences in the NK/T ratio were attributable to different distributions of single- and dual-lineage T/NK precursor clones. Because nearly all of the clones retained monocyte and/or granulocyte differentiation potentials in coculture with OP9-DL1 cells, T/NK precursors in PB are considered to be contained in the pool of T/NK/myeloid multipotent progenitors. The age-associated increase in NK over T cell commitment might occur in precursor cells with T/NK/myeloid potential. Copyright © 2013 by The American Association of Immunologists, Inc.
Journal Title:	Journal of Immunology
Volume:	190
Issue:	12
ISSN:	0022-1767
Publisher:	The American Association of Immunologists, Inc
Date Published:	2013-06-15
Start Page:	6164
End Page:	6172
Language:	English
DOI:	10.4049/jimmunol.1203189
PROVIDER:	scopus
PMCID:	PMC3679208
PUBMED:	23670190
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84879103597&partnerID=40&md5=f63494457e4414e68ac00a7b296ce119
Notes:	--- - "Export Date: 1 July 2013" - "CODEN: JOIMA" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/43255