Purification and partial characterization of a human hematopoietic precursor population Journal Article

  

Authors:	Smith, C.; Gasparetto, C.; Collins, N.; Gillio, A.; Muench, M. O.; O'Reilly, R. J.; Moore, M. A. S.
Article Title:	Purification and partial characterization of a human hematopoietic precursor population
Abstract:	This study reports the development of an assay, the Precolony-forming unit (CFU) assay, which detects human hematopoietic precursors. The Pre-CFU assay is based on the observation that precursors to CFU-granulocyte-macrophage (CFU-GM) that are undetectable in clonogenic assays differentiate into CFU-GM preferentially following treatment in suspension culture with recombinant human interleukin-1α (rhIL-1α) combined with rhIL-3. Using the Pre-CFU assay, hematopoietic precursors were detected in human bone marrow depleted of CFU-GM progenitors and differentiated hematopoietic elements via 4-hydroperoxycyclophosphamide treatment coupled with selection for CD34+ cells (4-HC(resistant)/CD34+ marrow). Additionally, the Pre-CFU assay detected recovery of hematopoiesis substantially earlier than the CFU-GM assay in primates following myeloablation with 5-flourouracil. The Pre-CFU assay was used to asses purification of a phenotypically defined hematopoietic precursor population, the lin-CD34+ population. The lin-CD34+ population lacks detectable surface markers for T-cell, B-cell, natural killer cell, and myeloid lineage, possesses the CD34 antigen, is devoid of CFU-GM progenitors, and yields Pre-CFU assay values comparable with 4-HC(resistant)/CD34+ marrow. Using a combination of phenotypic analysis and Pre-CFU assay analysis, the action of rhIL-1α plus rhIL-3 treatment on lin-CD34+ cells was further characterized. The data indicate that rhIL-1α plus rhIL-3 treatment induces proliferation and differentiation of early hematopoietic precursors into progenitors and terminally differentiated cells, without inducing a significant expansion of the precursor population itself.
Keywords:	adult; human tissue; human cell; fluorouracil; phenotype; animal; bone marrow cells; cell survival; cells, cultured; cell division; bone marrow; granulocyte macrophage colony stimulating factor; cyclophosphamide; cell differentiation; stem cell; cytokine; recombinant proteins; hematopoietic stem cells; interleukin 6; macrophages; macaca; colony-forming units assay; granulocyte colony stimulating factor; cell separation; antigens, cd34; intravenous drug administration; normal human; colony stimulating factor 1; colony forming unit gm; antigens, differentiation; recombinant interleukin 3; interleukin-3; granulocytes; human; male; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; recombinant interleukin 1alpha
Journal Title:	Blood
Volume:	77
Issue:	10
ISSN:	0006-4971
Publisher:	American Society of Hematology
Date Published:	1991-05-15
Start Page:	2122
End Page:	2128
Language:	English
PUBMED:	1709369
PROVIDER:	scopus
DOI:	10.1182/blood.V77.10.2122.2122
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025773505&partnerID=40&md5=70c5a5e5a0841ae86b96417d87c2adaa
Notes:	Article -- Source: Scopus

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