Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1ß Journal Article
| Authors: | Gasparetto, C.; Smith, C.; Gillio, A.; Stoppa, A. M.; Moore, M. A. S.; O'Reilly, R. J. |
| Article Title: | Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1ß |
| Abstract: | In a pre-clinical primate model, the effect of a single intravenous injection of rh-GM-CSF (50 mu g/kg), rh-IL3 (20 mu g/kg), rh-IL-1 beta (1 mu g/kg), rh-G-CSF (50 mu g/kg) and rh-pIXY 321 (50 mu g/kg) on peripheral blood mononuclear cell and hematopoietic stem cell concentration was determined, The results indicated that administration of a single dose of rh-IL-beta increased the concentrations of hematopoietic stem cells, including CFU-GM progenitors and precursors to CFU-GM, No toxicity was noted with this procedure. None of the remaining cytokines tested caused a significant increase in the peripheral blood hematopoietic stem cell concentration when administered in this manner and only G-CSF caused an increase in peripheral blood mononuclear cell concentrations, Autologous transplantation with limiting numbers of peripheral blood mononuclear cells (1X10(7) cells/kg) collected either 24 or 72 h following injection of 1 mu g/kg rh-IL-1 beta conferred a survival advantage to recipients compared with controls, These results imply that a single intravenous administration of rh-IL-1 beta may increase the concentration of peripheral blood stem cells to levels sufficient for transplantation. |
| Keywords: | chemotherapy; cyclophosphamide; transplantation; lymphoma; 5-fluorouracil; granulocyte; colony-stimulating factor; progenitor cells; interleukin-3; platelet recovery |
| Journal Title: | Bone Marrow Transplantation |
| Volume: | 14 |
| Issue: | 5 |
| ISSN: | 0268-3369 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1994-11-01 |
| Start Page: | 717 |
| End Page: | 723 |
| Language: | English |
| ACCESSION: | WOS:A1994PY42500005 |
| PROVIDER: | wos |
| PUBMED: | 7534159 |
| Notes: | Article -- Source: Wos |
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