Linkage between dendritic and T cell commitments in human circulating hematopoietic progenitors Journal Article

Authors: Kyoizumi, S.; Kubo, Y.; Kajimura, J.; Yoshida, K.; Hayashi, T.; Nakachi, K.; Young, L. F.; Moore, M. A.; van den Brink, M. R. M.; Kusunoki, Y.
Article Title: Linkage between dendritic and T cell commitments in human circulating hematopoietic progenitors
Abstract: The relationships between commitments of dendritic cells (DCs) and T cells in human hematopoietic stem cells are not well understood. In this study, we enumerate and characterize conventional DC and plasmacytoid DC precursors in association with T cell and thymus-derived types of NK cell precursors among CD34+ hematopoietic progenitor cells (HPCs) circulating in human peripheral blood. By limiting-dilution analyses using coculture with stroma cells expressing Notch1 ligand, the precursor frequencies (PFs) of DCs in HPCs were found to significantly correlate with T cell PFs, but not with NK cell PFs, among healthy donors. Clonal analyses showed that the majority of T/NK dual- and T single-lineage precursors-but only a minority of NK single-lineage precursors-were associated with the generation of DC progenies. All clones producing both DC and T cell progenies were found with monocyte and/or granulocyte progenies, suggesting DC differentiation via myeloid DC pathways. Analyses of peripheral blood HPC subpopulations revealed that the lineage split between DC and T/NK cell progenitor occurs at the stage prior to bifurcation into T and NK cell lineages. The findings suggest a strong linkage between DC and T cell commitments, which may be imprinted in circulating lymphoid-primed multipotent progenitors or in more upstream HPCs. Copyright © 2014 by The American Association of Immunologists, Inc. 0022-1767/14/$16.
Keywords: adult; controlled study; human cell; t lymphocyte; cd34 antigen; dendritic cell; cell differentiation; cell lineage; stem cell; pre t lymphocyte; cell subpopulation; hematopoietic stem cell; cell clone; stroma cell; correlational study; notch1 receptor; monocyte macrophage precursor cell; granulocyte precursor; human; priority journal; article; myeloid dendritic cell; dendritic progenitor cell; natural killer precursor cell
Journal Title: Journal of Immunology
Volume: 192
Issue: 12
ISSN: 0022-1767
Publisher: The American Association of Immunologists, Inc  
Date Published: 2014-06-01
Start Page: 5749
End Page: 5760
Language: English
DOI: 10.4049/jimmunol.1303260
PROVIDER: scopus
PMCID: PMC4048771
PUBMED: 24835400
Notes: J. Immunol. -- Export Date: 8 July 2014 -- CODEN: JOIMA -- Source: Scopus
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MSK Authors
  1. Malcolm A S Moore
    446 Moore
  2. Lauren Young
    39 Young