Induction of heat shock gene expression in RAT1 primary fibroblast cells by ELF electric fields Journal Article
| Authors: | Frisch, P.; Li, G. C.; McLeod, K.; Laramee, C. B. |
| Article Title: | Induction of heat shock gene expression in RAT1 primary fibroblast cells by ELF electric fields |
| Abstract: | Recent studies have demonstrated that the Ku70 gene fragment can be placed in the anti-sense orientation under the control of a heat-inducible heat shock protein 70 (HSP70) promoter and activated through heat shock exposure. This results in attenuation of the Ku70 protein expression, inhibiting cellular repair processes, and sensitizing the transfected cells to exposures such as the ionizing radiation exposures used clinically. However, achieving the tissue temperatures necessary to thermally induce the HSP70 response presents significant limitations to the clinical application of this strategy. Previous findings suggest an alternative approach to inducing a heat shock response, specifically through the use of extremely low frequency (ELF) electrical field stimulation. To further pursue this approach, we investigated HSP70 responses in transfected rat primary fibroblast (RAT1) cells exposed to 10Hz electric fields at intensities of 20-500V/m. We confirmed that low frequency electric fields can induce HSP70 heat shock expression, with peak responses obtained at 8h following a 2h field exposure. However, the approximate threefold increase in expression is substantially lower than that obtained using thermal stimulation, raising questions of the clinical utility of the response. © 2013 Wiley Periodicals, Inc. |
| Keywords: | dna binding protein; promoter region; genetics; dna-binding proteins; comparative study; radiation dose; animal; animals; transcription initiation; cell line; luciferase; validation study; transfection; radiation exposure; time; time factors; radiation dosage; genetic transfection; diagnostic agent; promoter regions, genetic; rat; fibroblast; cell culture techniques; fibroblasts; rats; heat shock protein 70; rattus; electromagnetic field; electromagnetic fields; ku antigen; culture technique; luciferases; transcriptional activation; hsp70 heat-shock proteins; antigens, nuclear; cell nucleus antigen; luminescent agents; electric field exposure; hsp70 expression; rat primary fibroblast (rat1) cells; luminescent agent |
| Journal Title: | Bioelectromagnetics |
| Volume: | 34 |
| Issue: | 5 |
| ISSN: | 0197-8462 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2013-07-01 |
| Start Page: | 405 |
| End Page: | 413 |
| Language: | English |
| DOI: | 10.1002/bem.21786 |
| PROVIDER: | scopus |
| PUBMED: | 23457046 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 July 2013" - "CODEN: BLCTD" - "Source: Scopus" |
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