Fluorescence correlation spectroscopy in living cells: A practical approach Journal Article
| Authors: | Altan-Bonnet, N.; Altan-Bonnet, G. |
| Article Title: | Fluorescence correlation spectroscopy in living cells: A practical approach |
| Abstract: | Fluorescence correlation spectroscopy (FCS) is a single-molecule fluorescence technique used to monitor molecular dynamics. It can be applied to living cells expressing fluorescently labeled proteins and lipids to determine the diffusion timescales and the total number of diffusing fluorescent molecules in the cell. A practical step-by-step approach to performing FCS with a commercial spectroscopy/microscopy system, the Zeiss Confocor 3, how to set up live-cell FCS experiments, acquire reliable data, and finally how to analyze the data acquired, are all described in this unit. © 2009 by John Wiley & Sons, Inc. |
| Keywords: | protein expression; review; research design; monitoring; cytology; animals; diffusion; fcs; fluorescence correlation spectroscopy; gfp; green fluorescent protein; live-cell imaging; cell protein; fluorescent dye; lipid; analytic method; bleaching; calibration; culture medium; molecular dynamics; signal noise ratio; cell line; fluorescent dyes; spectrometry, fluorescence |
| Journal Title: | Current Protocols in Cell Biology |
| Volume: | 45 |
| ISSN: | 1934-2500 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2009-12-01 |
| Start Page: | 4.24.1 |
| End Page: | 4.24.14 |
| Language: | English |
| DOI: | 10.1002/0471143030.cb0424s45 |
| PUBMED: | 20013755 |
| PROVIDER: | scopus |
| PMCID: | PMC3812671 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 30 November 2010" - "Source: Scopus" |
