Protocol to dissociate, process, and analyze the human lung tissue using single-cell RNA-seq Journal Article
| Authors: | Quintanal-Villalonga, Á; Chan, J. M.; Masilionis, I.; Gao, V. R.; Xie, Y.; Allaj, V.; Chow, A.; Poirier, J. T.; Pe'er, D.; Rudin, C. M.; Mazutis, L. |
| Article Title: | Protocol to dissociate, process, and analyze the human lung tissue using single-cell RNA-seq |
| Abstract: | We report a protocol for obtaining high-quality single-cell transcriptomics data from human lung biospecimens acquired from core needle biopsies, fine-needle aspirates, surgical resection, and pleural effusions. The protocol relies upon the brief mechanical and enzymatic disruption of tissue, enrichment of live cells by fluorescence-activated cell sorting (FACS), and droplet-based single-cell RNA sequencing (scRNA-seq). The protocol also details a procedure for analyzing the scRNA-seq data. For complete details on the use and execution of this protocol, please refer to Chan et al. (2021). © 2022 The Author(s) |
| Keywords: | sequence analysis; cell isolation; molecular biology; sequencing; single cell; cancer; rnaseq; flow cytometry/mass cytometry |
| Journal Title: | STAR Protocols |
| Volume: | 3 |
| Issue: | 4 |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press |
| Date Published: | 2022-12-16 |
| Start Page: | 101776 |
| Language: | English |
| DOI: | 10.1016/j.xpro.2022.101776 |
| PROVIDER: | scopus |
| PMCID: | PMC9597186 |
| PUBMED: | 36313536 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 November 2022 -- Source: Scopus |
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