Quantitative assessment of T cell repertoire recovery after hematopoietic stem cell transplantation Journal Article

   

Authors:	van Heijst, J. W. J.; Ceberio, I.; Lipuma, L. B.; Samilo, D. W.; Wasilewski, G. D.; Gonzales, A. M. R.; Nieves, J. L.; van den Brink, M. R. M.; Perales, M. A.; Pamer, E. G.
Article Title:	Quantitative assessment of T cell repertoire recovery after hematopoietic stem cell transplantation
Abstract:	Delayed T cell recovery and restricted T cell receptor (TCR) diversity after allogeneic hematopoietic stem cell transplantation (allo-HSCT) are associated with increased risks of infection and cancer relapse. Technical challenges have limited faithful measurement of TCR diversity after allo-HSCT. Here we combined 5′ rapid amplification of complementary DNA ends PCR with deep sequencing to quantify TCR diversity in 28 recipients of allo-HSCT using a single oligonucleotide pair. Analysis of duplicate blood samples confirmed that we accurately determined the frequency of individual TCRs. After 6 months, cord blood-graft recipients approximated the TCR diversity of healthy individuals, whereas recipients of T cell-depleted peripheral-blood stem cell grafts had 28-fold and 14-fold lower CD4+ and CD8+ T cell diversities, respectively. After 12 months, these deficiencies had improved for the CD4+ but not the CD8+ T cell compartment. Overall, this method provides unprecedented views of T cell repertoire recovery after allo-HSCT and may identify patients at high risk of infection or relapse. Copyright © 2013 Nature America, Inc.
Journal Title:	Nature Medicine
Volume:	19
Issue:	3
ISSN:	1078-8956
Publisher:	Nature Publishing Group
Date Published:	2013-03-01
Start Page:	372
End Page:	377
Language:	English
DOI:	10.1038/nm.3100
PROVIDER:	scopus
PMCID:	PMC3594333
PUBMED:	23435170
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84875228084&partnerID=40&md5=33a3b7c1474889f51aa8983eee59a003
Notes:	--- - "Export Date: 1 April 2013" - "CODEN: NAMEF" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/38939