The Smc5/6 complex and Esc2 influence multiple replication-associated recombination processes in Saccharomyces cerevisiae Journal Article
| Authors: | Choi, K.; Szakal, B.; Chen, Y. H.; Branzei, D.; Zhao, X. |
| Article Title: | The Smc5/6 complex and Esc2 influence multiple replication-associated recombination processes in Saccharomyces cerevisiae |
| Abstract: | Replication-associated recombinational repair is important for genome duplication and cell survival under DNA damage conditions. Several nonclassical recombination factors have been implicated in this process, but their functional relationships are not clear. Here, we show that three of these factors, Mph1, Mms2, and the Shu complex, can act independently to promote the formation of recombination intermediates during impaired replication. However, their functions become detrimental when cells lack the Smc5/6 complex or Esc2. We show that mph1Δ, mms2Δ, and shu1Δ suppress the sensitivity to the replicationblocking agent methylmethane sulfonate (MMS) in smc6 mutants, with double deletions conferring stronger suppression. These deletion mutations also rescue the MMS sensitivity of esc2Δ cells. In addition, two-dimensional gel analysis demonstrates that mph1Δ, mms2Δ, and shu1Δ each reduce the level of recombination intermediates in an smc6 mutant when cells replicate in the presence of MMS, and that double deletions lead to a greater reduction. Our work thus suggests that Mph1, Mms2, and the Shu complex can function in distinct pathways in replication-associated recombinational repair and that the Smc5/6 complex and Esc2 prevent the accumulation of toxic recombination intermediates generated in these processes. © 2010 by The American Society for Cell Biology. |
| Keywords: | controlled study; unclassified drug; nonhuman; dna replication; protein function; cell cycle proteins; dna damage; cell survival; cell division; dna repair; cell protein; nuclear proteins; genetic recombination; saccharomyces cerevisiae; recombination, genetic; gene duplication; saccharomyces cerevisiae proteins; deletion mutant; gene replication; mesylic acid methyl ester; protein esc2; protein mms2; protein mph1; protein shu; protein smc5 6; sulfonic acid derivative |
| Journal Title: | Molecular Biology of the Cell |
| Volume: | 21 |
| Issue: | 13 |
| ISSN: | 1059-1524 |
| Publisher: | The American Society for Cell Biology |
| Date Published: | 2010-07-01 |
| Start Page: | 2306 |
| End Page: | 2314 |
| Language: | English |
| DOI: | 10.1091/mbc.E10-01-0050 |
| PUBMED: | 20444977 |
| PROVIDER: | scopus |
| PMCID: | PMC2893993 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: MBCEE" - "Source: Scopus" |
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