Interplay between the Smc5/6 complex and the Mph1 helicase in recombinational repair Journal Article
| Authors: | Chen, Y. H.; Choi, K.; Szakal, B.; Arenz, J.; Duan, X.; Ye, H.; Branzei, D.; Zhao, X. |
| Article Title: | Interplay between the Smc5/6 complex and the Mph1 helicase in recombinational repair |
| Abstract: | The evolutionarily conserved Smc5/6 complex is implicated in recombinational repair, but its function in this process has been elusive. Here we report that the budding yeast Smc5/6 complex directly binds to the DNA helicase Mph1. Mph1 and its helicase activity define a replication-associated recombination subpathway. We show that this pathway is toxic when the Smc5/6 complex is defective, because mph1△ and its helicase mutations suppress multiple defects in mutants of the Smc5/6 complex, including their sensitivity to replication-blocking agents, growth defects, and inefficient chromatid separation, whereas MPH1 overexpression exacerbates some of these defects. We further demonstrate that Mph1 and its helicase activity are largely responsible for the accumulation of potentially deleterious recombination intermediates in mutants of the Smc5/6 complex. We also present evidence that mph1△ does not alleviate sensitivity to DNA damage or the accumulation of recombination intermediates in cells lacking Sgs1, which is thought to function together with the Smc5/6 complex. Thus, our results reveal a function of the Smc5/6 complex in the Mph1-dependent recombinational subpathway that is distinct from Sgs1. We suggest that the Smc5/6 complex can counteract/modulate a pro-recombinogenic function of Mph1 or facilitate the resolution of recombination structures generated by Mph1. |
| Keywords: | unclassified drug; gene mutation; nonhuman; protein function; cell cycle proteins; dna damage; dna repair; gene overexpression; protein; enzyme activity; dead-box rna helicases; dna; recombination, genetic; gene interaction; recombinant protein; helicase; structural maintenance of chromosome 5; structural maintenance of chromosome 6; budding; yeast; mutant proteins; recq helicases; saccharomyces cerevisiae proteins; saccharomycetales |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 106 |
| Issue: | 50 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2009-12-15 |
| Start Page: | 21252 |
| End Page: | 21257 |
| Language: | English |
| DOI: | 10.1073/pnas.0908258106 |
| PUBMED: | 19995966 |
| PROVIDER: | scopus |
| PMCID: | PMC2795505 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 3" - "Export Date: 30 November 2010" - "CODEN: PNASA" - "Source: Scopus" |
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