Designs and concept reliance of a fully automated high-content screening platform Journal Article
| Authors: | Radu, C.; Adrar, H. S.; Alamir, A.; Hatherley, I.; Trinh, T.; Djaballah, H. |
| Article Title: | Designs and concept reliance of a fully automated high-content screening platform |
| Abstract: | High-content screening (HCS) is becoming an accepted platform in academic and industry screening labs and does require slightly different logistics for execution. To automate our stand-alone HCS microscopes, namely, an alpha IN Cell Analyzer 3000 (INCA3000), originally a Praelux unit hooked to a Hudson Plate Crane with a maximum capacity of 50 plates per run, and the IN Cell Analyzer 2000 (INCA2000), in which up to 320 plates could be fed per run using the Thermo Fisher Scientific Orbitor, we opted for a 4 m linear track system harboring both microscopes, plate washer, bulk dispensers, and a high-capacity incubator allowing us to perform both live and fixed cell-based assays while accessing both microscopes on deck. Considerations in design were given to the integration of the alpha INCA3000, a new gripper concept to access the onboard nest, and peripheral locations on deck to ensure a self-reliant system capable of achieving higher throughput. The resulting system, referred to as Hestia, has been fully operational since the new year, has an onboard capacity of 504 plates, and harbors the only fully automated alpha INCA3000 unit in the world. |
| Keywords: | microscopy; microscopes; methodology; cytology; high throughput screening; drug screening; drug evaluation, preclinical; high-throughput screening assays; automation; cytological techniques; cell-based assays; cells; high-content screening; hts; laboratory automation; hcs; automation, laboratory; in cell analyzer 3000; in cell analyzer 2000; fully automated; fully operational; high-capacity; linear tracks |
| Journal Title: | Journal of Laboratory Automation |
| Volume: | 17 |
| Issue: | 5 |
| ISSN: | 2211-0682 |
| Publisher: | Society for Laboratory Automation and Screening |
| Date Published: | 2012-10-01 |
| Start Page: | 359 |
| End Page: | 369 |
| Language: | English |
| PUBMED: | 22797489 |
| PROVIDER: | scopus |
| PMCID: | PMC3626108 |
| DOI: | 10.1177/2211068212453311 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 March 2013" - "Source: Scopus" |
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