Adapting human pluripotent stem cells to high-throughput and high-content screening Journal Article
| Authors: | Desbordes, S. C.; Studer, L. |
| Article Title: | Adapting human pluripotent stem cells to high-throughput and high-content screening |
| Abstract: | The increasing use of human pluripotent stem cells (hPSCs) as a source of cells for drug discovery, cytotoxicity assessment and disease modeling requires their adaptation to large-scale culture conditions and screening formats. Here, we describe a simple and robust protocol for the adaptation of human embryonic stem cells (hESCs) to high-throughput screening (HTS). This protocol can also be adapted to human induced pluripotent stem cells (hiPSCs) and high-content screening (HCS). We also describe a 7-d assay to identify compounds with an effect on hESC self-renewal and differentiation. This assay can be adapted to a variety of applications. The procedure involves the culture expansion of hESCs, their adaptation to 384-well plates, the addition of small molecules or other factors, and finally data acquisition and processing. In this protocol, the optimal number of hESCs plated in 384-well plates has been adapted to HTS/HCS assays of 7 d. © 2012 Nature America, Inc. All rights reserved. |
| Journal Title: | Nature Protocols |
| Volume: | 8 |
| Issue: | 1 |
| ISSN: | 1754-2189 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2013-01-01 |
| Start Page: | 111 |
| End Page: | 130 |
| Language: | English |
| DOI: | 10.1038/nprot.2012.139 |
| PROVIDER: | scopus |
| PUBMED: | 23257981 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 February 2013" - "Source: Scopus" |
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