Adapting human pluripotent stem cells to high-throughput and high-content screening Journal Article


Authors: Desbordes, S. C.; Studer, L.
Article Title: Adapting human pluripotent stem cells to high-throughput and high-content screening
Abstract: The increasing use of human pluripotent stem cells (hPSCs) as a source of cells for drug discovery, cytotoxicity assessment and disease modeling requires their adaptation to large-scale culture conditions and screening formats. Here, we describe a simple and robust protocol for the adaptation of human embryonic stem cells (hESCs) to high-throughput screening (HTS). This protocol can also be adapted to human induced pluripotent stem cells (hiPSCs) and high-content screening (HCS). We also describe a 7-d assay to identify compounds with an effect on hESC self-renewal and differentiation. This assay can be adapted to a variety of applications. The procedure involves the culture expansion of hESCs, their adaptation to 384-well plates, the addition of small molecules or other factors, and finally data acquisition and processing. In this protocol, the optimal number of hESCs plated in 384-well plates has been adapted to HTS/HCS assays of 7 d. © 2012 Nature America, Inc. All rights reserved.
Journal Title: Nature Protocols
Volume: 8
Issue: 1
ISSN: 1754-2189
Publisher: Nature Publishing Group  
Date Published: 2013-01-01
Start Page: 111
End Page: 130
Language: English
DOI: 10.1038/nprot.2012.139
PROVIDER: scopus
PUBMED: 23257981
DOI/URL:
Notes: --- - "Export Date: 1 February 2013" - "Source: Scopus"
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  1. Lorenz Studer
    220 Studer