RNase III-independent microRNA biogenesis in mammalian cells Journal Article
| Authors: | Maurin, T.; Cazalla, D.; Yang, J. S.; Bortolamiol-Becet, D.; Lai, E. C. |
| Article Title: | RNase III-independent microRNA biogenesis in mammalian cells |
| Abstract: | RNase III enzymes are fundamental to the biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs) in all species studied. Although alternative miRNA pathways independent of Drosha or Dicer exist, each still requires one RNase III-type enzyme. Here, we describe two strategies that marry either RNase Z or the Integrator complex with the slicing activity of Argonaute2 to generate highly functional mature miRNAs. We provide stringent validation of their RNase III independence by demonstrating efficient miRNA biogenesis and activity in Drosha and Dicer knockout cells. These data provide proof-of-principle evidence for additional mechanistic possibilities for efficient generation of small regulatory RNAs, and represent novel silencing triggers that may be exploited for technical purposes. Copyright © 2012 RNA Society. |
| Keywords: | protein expression; nonhuman; animal; animals; mice; complex formation; microrna; cell line; small interfering rna; rna interference; hela cells; dead-box rna helicases; immunoprecipitation; base sequence; micrornas; rna, viral; biogenesis; dicer; ribonuclease iii; argonaute 2 protein; endoribonucleases; argonaute; hek293 cells; gene knockout techniques; slicer; rna, small nuclear; argonaute proteins; rnase iii; herpesvirus 2, saimiriine |
| Journal Title: | RNA |
| Volume: | 18 |
| Issue: | 12 |
| ISSN: | 1355-8382 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2012-12-01 |
| Start Page: | 2166 |
| End Page: | 2173 |
| Language: | English |
| DOI: | 10.1261/rna.036194.112 |
| PROVIDER: | scopus |
| PMCID: | PMC3504669 |
| PUBMED: | 23097423 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 January 2013" - "CODEN: RNARF" - "Source: Scopus" |
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