RecA-binding pilE G4 sequence essential for pilin antigenic variation forms monomeric and 5′ end-stacked dimeric parallel G-quadruplexes Journal Article
| Authors: | Kuryavyi, V.; Cahoon, L. A.; Seifert, H. S.; Patel, D. J. |
| Article Title: | RecA-binding pilE G4 sequence essential for pilin antigenic variation forms monomeric and 5′ end-stacked dimeric parallel G-quadruplexes |
| Abstract: | Neisseria gonorrhoeae is an obligate human pathogen that can escape immune surveillance through antigenic variation of surface structures such as pili. A G-quadruplex-forming (G4) sequence (5′-G3TG3TTG 3TG3) located upstream of the N. gonorrhoeae pilin expression locus (pilE) is necessary for initiation of pilin antigenic variation, a recombination-based, high-frequency, diversity-generation system. We have determined NMR-based structures of the all parallel-stranded monomeric and 5′ end-stacked dimeric pilE G-quadruplexes in monovalent cation-containing solutions. We demonstrate that the three-layered all parallel-stranded monomeric pilE G-quadruplex containing single-residue double-chain reversal loops, which can be modeled without steric clashes into the 3 nt DNA-binding site of RecA, binds and promotes E. coli RecA-mediated strand exchange in vitro. We discuss how interactions between RecA and monomeric pilE G-quadruplex could facilitate the specialized recombination reactions leading to pilin diversification. © 2012 Elsevier Ltd. |
| Journal Title: | Structure |
| Volume: | 20 |
| Issue: | 12 |
| ISSN: | 0969-2126 |
| Publisher: | Cell Press |
| Date Published: | 2012-12-05 |
| Start Page: | 2090 |
| End Page: | 2102 |
| Language: | English |
| DOI: | 10.1016/j.str.2012.09.013 |
| PROVIDER: | scopus |
| PUBMED: | 23085077 |
| PMCID: | PMC3845372 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 January 2013" - "CODEN: STRUE" - "Source: Scopus" |
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