Kinetics of double-chain reversals bridging contiguous quartets in tetramolecular quadruplexes Journal Article
| Authors: | Mergny, J. L.; De Cian, A.; Amrane, S.; da Silva, M. W. |
| Article Title: | Kinetics of double-chain reversals bridging contiguous quartets in tetramolecular quadruplexes |
| Abstract: | Repetitive 5′GGXGGDNA segments abound in, or near, regulatory regions of the genome and may form unusual structures called G-quadruplexes. Using NMR spectroscopy, we demonstrate that a family of 5′GCGGXGGY sequences adopts a folding topology containing double-chain reversals. The topology is composed of two bistranded quadruplex monomeric units linked by formation of G:C:G:C tetrads. We provide a complete thermodynamic and kinetic analysis of 13 different sequences using absorbance spectroscopy and DSC, and compare their kinetics with a canonical tetrameric parallel-stranded quadruplex formed by TG4T. We demonstrate large differences up to 105-fold) in the association constants of these quadruplexes depending on primary sequence; the fastest samples exhibiting association rate equal or higher than the canonical TG4T quadruplex. In contrast, all sequences studied here unfold at a lower temperature than this quadruplex. Some sequences have thermodynamic stability comparable to the canonical TG4T tetramolecular quadruplex, but with faster association and dissociation. Sequence effects on the dissociation processes are discussed in light of structural data. © 2006 Oxford University Press. |
| Keywords: | promoter region; ph; chemistry; dna; kinetics; guanine; nucleotide sequence; nuclear magnetic resonance spectroscopy; base sequence; genome; temperature; thermodynamics; nomenclature; conformation; nucleic acid conformation; oligonucleotide; nuclear magnetic resonance; hydrogen-ion concentration; nucleic acid structure; nuclear magnetic resonance, biomolecular; differential scanning calorimetry; low temperature; nucleotide metabolism; quadruplex dna; osmolarity; calorimetry, differential scanning; osmolar concentration |
| Journal Title: | Nucleic Acids Research |
| Volume: | 34 |
| Issue: | 8 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 2006-01-01 |
| Start Page: | 2386 |
| End Page: | 2397 |
| Language: | English |
| DOI: | 10.1093/nar/gkl098 |
| PUBMED: | 16682446 |
| PROVIDER: | scopus |
| PMCID: | PMC1458523 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 13" - "Export Date: 4 June 2012" - "CODEN: NARHA" - "Source: Scopus" |
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