Structure of the SWI2/SNF2 chromatin-remodeling domain of eukaryotic Rad54 Journal Article

Authors: Thomä, N. H.; Czyzewski, B. K.; Alexeev, A. A.; Mazin, A. V.; Kowalczykowski, S. C.; Pavletich, N. P.
Article Title: Structure of the SWI2/SNF2 chromatin-remodeling domain of eukaryotic Rad54
Abstract: SWI2/SNF2 chromatin-remodeling proteins mediate the mobilization of nucleosomes and other DNA-associated proteins. SWI2/SNF2 proteins contain sequence motifs characteristic of SF2 helicases but do not have helicase activity. Instead, they couple ATP hydrolysis with the generation of superhelical torsion in DNA. The structure of the nucleosome-remodeling domain of zebrafish Rad54, a protein involved in Rad51-mediated homologous recombination, reveals that the core of the SWI2/SNF2 enzymes consist of two α/β-lobes similar to SF2 helicases. The Rad54 helicase lobes contain insertions that form two helical domains, one within each lobe. These insertions contain SWI2/SNF2-specific sequence motifs likely to be central to SWI2/SNF2 function. A broad cleft formed by the two lobes and flanked by the helical insertions contains residues conserved in SWI2/SNF2 proteins and motifs implicated in DNA-binding by SF2 helicases. The Rad54 structure suggests that SWI2/SNF2 proteins use a mechanism analogous to helicases to translocate on dsDNA.
Keywords: controlled study; carrier protein; unclassified drug; dna binding protein; sequence analysis; genetics; dna-binding proteins; nonhuman; molecular genetics; protein domain; protein function; protein motif; animal; metabolism; animals; homologous recombination; cell line; transcription factor; enzyme activity; transcription factors; chemistry; amino acid sequence; molecular sequence data; sequence alignment; eukaryota; chromatin; nucleotide sequence; rad54 protein; models, molecular; protein structure, tertiary; chemical structure; helicase; adenosine triphosphate; hydrolysis; protein tertiary structure; dna helicases; zebra fish; zebrafish; eukaryote; amino acid motifs; chromatin assembly and disassembly; rad51 protein; danio rerio; nucleosome; crystallography; dna supercoiling; protein swi; zebrafish proteins; deoxyribonucleoprotein; protein snf2; zebrafish protein; rad54 protein, zebrafish; smarca1 protein, human
Journal Title: Nature Structural and Molecular Biology
Volume: 12
Issue: 4
ISSN: 1545-9993
Publisher: Nature Publishing Group  
Date Published: 2005-04-01
Start Page: 350
End Page: 356
Language: English
DOI: 10.1038/nsmb919
PUBMED: 15806108
PROVIDER: scopus
Notes: --- - "Cited By (since 1996): 84" - "Export Date: 24 October 2012" - "CODEN: NSMBC" - "Molecular Sequence Numbers: GENBANK: AAC97986, NP_060023, P28370, Q03468, Q12873;" - "Source: Scopus"
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MSK Authors
  1. Nicolas Thoma
    5 Thoma