Rad51 presynaptic filament stabilization function of the mouse Swi5-Sfr1 heterodimeric complex Journal Article


Authors: Tsai, S. P.; Su, G. C.; Lin, S. W.; Chung, C. I.; Xue, X.; Dunlop, M. H.; Akamatsu, Y.; Jasin, M.; Sung, P.; Chi, P.
Article Title: Rad51 presynaptic filament stabilization function of the mouse Swi5-Sfr1 heterodimeric complex
Abstract: Homologous recombination (HR) represents a major error-free pathway to eliminate pre-carcinogenic chromosomal lesions. The DNA strand invasion reaction in HR is mediated by a helical filament of the Rad51 recombinase assembled on single-stranded DNA that is derived from the nucleolytic processing of the primary lesion. Recent studies have found that the human and mouse Swi5 and Sfr1 proteins form a complex that influences Rad51-mediated HR in cells. Here, we provide biophysical evidence that the mouse Swi5-Sfr1 complex has a 1:1 stoichiometry. Importantly, the Swi5-Sfr1 complex, but neither Swi5 nor Sfr1 alone, physically interacts with Rad51 and stimulates Rad51-mediated homologous DNA pairing. This stimulatory effect stems from the stabilization of the Rad51-ssDNA presynaptic filament. Moreover, we provide evidence that the RSfp (rodent Sfr1 proline rich) motif in Sfr1 serves as a negative regulatory element. These results thus reveal an evolutionarily conserved function in the Swi5-Sfr1 complex and furnish valuable information as to the regulatory role of the RSfp motif that isspecific to themammalianSfr1 orthologs. © The Author(s) 2012.
Journal Title: Nucleic Acids Research
Volume: 40
Issue: 14
ISSN: 0305-1048
Publisher: Oxford University Press  
Date Published: 2012-08-01
Start Page: 6558
End Page: 6569
Language: English
DOI: 10.1093/nar/gks305
PROVIDER: scopus
PMCID: PMC3413116
PUBMED: 22492707
DOI/URL:
Notes: --- - "Export Date: 4 September 2012" - "CODEN: NARHA" - "Source: Scopus"
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MSK Authors
  1. Maria Jasin
    210 Jasin