Rad51 presynaptic filament stabilization function of the mouse Swi5-Sfr1 heterodimeric complex Journal Article
| Authors: | Tsai, S. P.; Su, G. C.; Lin, S. W.; Chung, C. I.; Xue, X.; Dunlop, M. H.; Akamatsu, Y.; Jasin, M.; Sung, P.; Chi, P. |
| Article Title: | Rad51 presynaptic filament stabilization function of the mouse Swi5-Sfr1 heterodimeric complex |
| Abstract: | Homologous recombination (HR) represents a major error-free pathway to eliminate pre-carcinogenic chromosomal lesions. The DNA strand invasion reaction in HR is mediated by a helical filament of the Rad51 recombinase assembled on single-stranded DNA that is derived from the nucleolytic processing of the primary lesion. Recent studies have found that the human and mouse Swi5 and Sfr1 proteins form a complex that influences Rad51-mediated HR in cells. Here, we provide biophysical evidence that the mouse Swi5-Sfr1 complex has a 1:1 stoichiometry. Importantly, the Swi5-Sfr1 complex, but neither Swi5 nor Sfr1 alone, physically interacts with Rad51 and stimulates Rad51-mediated homologous DNA pairing. This stimulatory effect stems from the stabilization of the Rad51-ssDNA presynaptic filament. Moreover, we provide evidence that the RSfp (rodent Sfr1 proline rich) motif in Sfr1 serves as a negative regulatory element. These results thus reveal an evolutionarily conserved function in the Swi5-Sfr1 complex and furnish valuable information as to the regulatory role of the RSfp motif that isspecific to themammalianSfr1 orthologs. © The Author(s) 2012. |
| Journal Title: | Nucleic Acids Research |
| Volume: | 40 |
| Issue: | 14 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 2012-08-01 |
| Start Page: | 6558 |
| End Page: | 6569 |
| Language: | English |
| DOI: | 10.1093/nar/gks305 |
| PROVIDER: | scopus |
| PMCID: | PMC3413116 |
| PUBMED: | 22492707 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 4 September 2012" - "CODEN: NARHA" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work