Spontaneous proliferation and type 2 cytokine secretion by CD4+T cells in patients with metastatic melanoma vaccinated with antigen-pulsed dendritic cells Journal Article
| Authors: | Palucka, A. K.; Connolly, J.; Ueno, H.; Kohl, J.; Paczesny, S.; Dhodapkar, M.; Fay, J.; Banchereau, J. |
| Article Title: | Spontaneous proliferation and type 2 cytokine secretion by CD4+T cells in patients with metastatic melanoma vaccinated with antigen-pulsed dendritic cells |
| Abstract: | We observed the induction of spontaneous, i.e., without adding exogenous antigen, in vitro proliferation of PBMCs from patients with stage IV melanoma who underwent repeated vaccinations with antigen-loaded dendritic cells (DCs) derived from CD34+ hematopoietic progenitors (CD34-DCs). Proliferating cells are CD4+T cells. Their proliferation is dependent on (1) CD11c+ myeloid DCs, since their depletion from PBMCs abolishes it; and (2) IL-2, as it can be blocked by neutralizing anti-IL-2 antibodies. Spontaneous proliferation is associated to the secretion of type 2 cytokines. To analyze the frequency of spontaneous proliferation induction in the cohort of 18 vaccinated patients, an index of spontaneous proliferation was defined as a ratio of PBMCs proliferation from post- vs pre-DC vaccination blood samples. Ten out of sixteen analyzed patients showed an index >2. The index of spontaneous proliferation correlates with antigen-specific PBMC proliferation to the vaccine antigen KLH. Furthermore, both spontaneous- and antigen-specific proliferation in PBMC cultures are dependent on blood myeloid DCs. © 2005 Springer Science+Business Media, Inc. |
| Keywords: | clinical article; controlled study; human cell; cancer staging; cell proliferation; lymphocyte proliferation; t lymphocyte; cells, cultured; cd34 antigen; interleukin 2; melanoma; metastasis; dendritic cell; protein depletion; cytokine; antigen presentation; cytokines; dendritic cells; correlation analysis; cellular immunity; human cell culture; cancer vaccine; cd4-positive t-lymphocytes; vaccination; neoplasm metastasis; tumor immunity; bone marrow cell; hematopoietic stem cell; cd4 antigen; cytokine release; cell communication; immunotherapy, adoptive; peripheral blood mononuclear cell; cell secretion; keyhole limpet hemocyanin; hemocyanin; cd11 antigen; antigens, cd11c; revaccination; t cell immunity |
| Journal Title: | Journal of Clinical Immunology |
| Volume: | 25 |
| Issue: | 3 |
| ISSN: | 0271-9142 |
| Publisher: | Springer |
| Date Published: | 2005-05-01 |
| Start Page: | 288 |
| End Page: | 295 |
| Language: | English |
| DOI: | 10.1007/s10875-005-4089-z |
| PUBMED: | 15981095 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 8" - "Export Date: 24 October 2012" - "CODEN: JCIMD" - "Source: Scopus" |
