Antigen-bearing immature dendritic cells induce peptide-specific CD8+ regulatory T cells in vivo in humans Journal Article
| Authors: | Dhodapkar, M. V.; Steinman, R. M. |
| Article Title: | Antigen-bearing immature dendritic cells induce peptide-specific CD8+ regulatory T cells in vivo in humans |
| Abstract: | Regulatory T cells (TRs) can suppress the function of other effector T cells in the setting of autoimmunity, transplantation, and resistance to tumors. The mechanism for the induction of TRs has not been defined. We previously reported that an njection of immature dendritic cells (DCs) pulsed with influenza matrix peptide (MP) led 7 days later to antigen-specific silencing of effector T-cell function in the blood of 2 healthy human subjects. Here, we found that interferon-γ-producing effectors return by 6 months. Importantly, in mixing experiments, CD8+ T cells from the sample obtained 7 days after injection could suppress MP-specific effectors obtained before injection and those in recovery samples. This suppression or regulation was specific for the immunizing peptide (MP) and cell-dose dependent, and it required contact between the 2 samples. These data show the capacity of immature DCs to induce antigen-specific regulatory CD8+ T cells in humans. © 2002 by The American Society of Hematology. |
| Keywords: | signal transduction; controlled study; human cell; cd8 antigen; cd8-positive t-lymphocytes; cell function; cell maturation; dendritic cell; in vivo study; immunoregulation; regulatory t lymphocyte; antigen presentation; dendritic cells; immune response; regulatory mechanism; gamma interferon; antigen specificity; peptide fragments; adoptive transfer; cytokine production; effector cell; matrix protein; t lymphocyte subpopulation; viral matrix proteins; t lymphocyte activation; concentration response; cell transplantation; normal human; human experiment; interleukin-10; cell kinetics; interferon type ii; humans; human; priority journal; article |
| Journal Title: | Blood |
| Volume: | 100 |
| Issue: | 1 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2002-07-01 |
| Start Page: | 174 |
| End Page: | 177 |
| Language: | English |
| DOI: | 10.1182/blood.V100.1.174 |
| PUBMED: | 12070024 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
