Conventional liver CD4 T cells are functionally distinct and suppressed by environmental factors Journal Article
| Authors: | Katz, S. C.; Pillarisetty, V. G.; Bleier, J. I.; Kingham, T. P.; Chaudhry, U. I.; Shah, A. B.; DeMatteo, R. P. |
| Article Title: | Conventional liver CD4 T cells are functionally distinct and suppressed by environmental factors |
| Abstract: | The contribution of intrahepatic conventional T cells to the unique immunologic properties of the liver has not been clearly defined. We isolated bulk and CD4 T cells from mouse liver and compared their functions with each other and with their splenic counterparts. Unlike bulk spleen T cells, bulk liver T cells reacted minimally to allogeneic or antigen-loaded syngeneic dendritic cells. However, after exclusion of natural killer T cells (NKTs) and γδ T cells by FACS, liver and spleen CD4 T cells actually proliferated to a similar extent upon allogeneic or antigen-specific stimulation. Liver CD4 T cells were more sensitive to interleukin 2 (IL-2) than were spleen CD4 T cells, but had a similar proliferative potential based on their response to CD3 ligation. In addition, activated liver CD4 T cells produced higher levels of IL-4, IL-5, IL-10, and interferon gamma (IFN-γ) than did splenic CD4 T cells. Therefore, liver CD4 T cells are intrinsically different from spleen CD4 T cells. In vitro, liver or spleen NKTs and γδ T cells suppressed liver and spleen CD4 T-cell proliferation in a dose-dependent fashion. In conclusion, unconventional T cells constrain liver CD4 T-cell function. Our findings have implications for pathological conditions of the liver that involve the response of conventional CD4 T lymphocytes. Copyright © 2005 by the American Association for the Study of Liver Diseases. |
| Keywords: | controlled study; nonhuman; cd3 antigen; lymphocyte proliferation; t lymphocyte; animal cell; mouse; animals; mice; interleukin 2; spleen; interleukin 10; interleukin 4; interleukin 5; mice, inbred balb c; mice, inbred c57bl; liver; lymphocyte activation; cytokines; dendritic cells; immune tolerance; gamma interferon; cd4-positive t-lymphocytes; natural killer cell; cytokine production; cell stimulation; environmental factor; fluorescence activated cell sorting; lymphocyte function; interleukin-2; spleen cell; receptors, antigen, t-cell, gamma-delta; cd4-cd8 ratio |
| Journal Title: | Hepatology |
| Volume: | 42 |
| Issue: | 2 |
| ISSN: | 0270-9139 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2005-08-01 |
| Start Page: | 293 |
| End Page: | 300 |
| Language: | English |
| DOI: | 10.1002/hep.20795 |
| PUBMED: | 16025518 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 8" - "Export Date: 24 October 2012" - "CODEN: HPTLD" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work