Conventional liver CD4 T cells are functionally distinct and suppressed by environmental factors Journal Article


Authors: Katz, S. C.; Pillarisetty, V. G.; Bleier, J. I.; Kingham, T. P.; Chaudhry, U. I.; Shah, A. B.; DeMatteo, R. P.
Article Title: Conventional liver CD4 T cells are functionally distinct and suppressed by environmental factors
Abstract: The contribution of intrahepatic conventional T cells to the unique immunologic properties of the liver has not been clearly defined. We isolated bulk and CD4 T cells from mouse liver and compared their functions with each other and with their splenic counterparts. Unlike bulk spleen T cells, bulk liver T cells reacted minimally to allogeneic or antigen-loaded syngeneic dendritic cells. However, after exclusion of natural killer T cells (NKTs) and γδ T cells by FACS, liver and spleen CD4 T cells actually proliferated to a similar extent upon allogeneic or antigen-specific stimulation. Liver CD4 T cells were more sensitive to interleukin 2 (IL-2) than were spleen CD4 T cells, but had a similar proliferative potential based on their response to CD3 ligation. In addition, activated liver CD4 T cells produced higher levels of IL-4, IL-5, IL-10, and interferon gamma (IFN-γ) than did splenic CD4 T cells. Therefore, liver CD4 T cells are intrinsically different from spleen CD4 T cells. In vitro, liver or spleen NKTs and γδ T cells suppressed liver and spleen CD4 T-cell proliferation in a dose-dependent fashion. In conclusion, unconventional T cells constrain liver CD4 T-cell function. Our findings have implications for pathological conditions of the liver that involve the response of conventional CD4 T lymphocytes. Copyright © 2005 by the American Association for the Study of Liver Diseases.
Keywords: controlled study; nonhuman; cd3 antigen; lymphocyte proliferation; t lymphocyte; animal cell; mouse; animals; mice; interleukin 2; spleen; interleukin 10; interleukin 4; interleukin 5; mice, inbred balb c; mice, inbred c57bl; liver; lymphocyte activation; cytokines; dendritic cells; immune tolerance; gamma interferon; cd4-positive t-lymphocytes; natural killer cell; cytokine production; cell stimulation; environmental factor; fluorescence activated cell sorting; lymphocyte function; interleukin-2; spleen cell; receptors, antigen, t-cell, gamma-delta; cd4-cd8 ratio
Journal Title: Hepatology
Volume: 42
Issue: 2
ISSN: 0270-9139
Publisher: John Wiley & Sons  
Date Published: 2005-08-01
Start Page: 293
End Page: 300
Language: English
DOI: 10.1002/hep.20795
PUBMED: 16025518
PROVIDER: scopus
DOI/URL:
Notes: --- - "Cited By (since 1996): 8" - "Export Date: 24 October 2012" - "CODEN: HPTLD" - "Source: Scopus"
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MSK Authors
  1. Ronald P DeMatteo
    609 DeMatteo
  2. Steven C Katz
    33 Katz
  3. T Peter Kingham
    297 Kingham
  4. Joshua Israel Bleier
    13 Bleier
  5. Alaap Shah
    20 Shah