Liver dendritic cells are less immunogenic than spleen dendritic cells because of differences in subtype composition Journal Article

  

Authors:	Pillarisetty, V. G.; Shah, A. B.; Miller, G.; Bleier, J. I.; DeMatteo, R. P.
Article Title:	Liver dendritic cells are less immunogenic than spleen dendritic cells because of differences in subtype composition
Abstract:	The unique immunological properties of the liver may be due to the function of hepatic dendritic cells (DC). However, liver DC have not been well characterized because of the difficulty in isolating adequate numbers of cells for analysis. Using immunomagnetic bead and flow cytometric cell sorting, we compared freshly isolated murine liver and spleen CD11c+ DC. We found that liver DC are less mature, capture less Ag, and induce less T cell stimulation than spleen DC. Nevertheless, liver DC were able to generate high levels of IL-12 in response to CpG stimulation. We identified four distinct subtypes of liver DC based on the widely used DC subset markers CD8α and CD11b. Lymphoid (CD8α+CD11b-) and myeloid (CD8α-CD11b+) liver DC activated T cells to a similar degree as did their splenic DC counterparts but comprised only 20% of all liver DC. In contrast, the two more prevalent liver DC subsets were only weakly immunostimulatory. Plasmacytoid DC (B220+) accounted for 19% of liver DC, but only 5% of spleen DC. Our findings support the widely held notion that liver DC are generally weak activators of immunity, although they are capable of producing inflammatory cytokines, and certain subtypes potently activate T cells.
Keywords:	immunohistochemistry; allograft; nonhuman; flow cytometry; cd8 antigen; animal cell; mouse; animals; mice; animal tissue; spleen; dendritic cell; cell differentiation; mice, inbred balb c; mice, inbred c57bl; stem cell; liver; antigen presentation; lymphocyte activation; dendritic cells; antigens; immunogenicity; cd11b antigen; autoimmunity; immunophenotyping; t-lymphocyte subsets; liver cell; cytokine release; liver parenchyma; t lymphocyte activation; interleukin 12; spleen cell; antigens, cd8; antigens, cd11b; liver graft; ovalbumin; male; priority journal; article
Journal Title:	Journal of Immunology
Volume:	172
Issue:	2
ISSN:	0022-1767
Publisher:	The American Association of Immunologists, Inc
Date Published:	2004-01-15
Start Page:	1009
End Page:	1017
Language:	English
PROVIDER:	scopus
PUBMED:	14707074
DOI:	10.4049/​jimmunol.172.2.1009
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-1642495750&partnerID=40&md5=2fa019a85ec3247f9fd49eda3588e91b
Notes:	J. Immunol. -- Cited By (since 1996):109 -- Export Date: 16 June 2014 -- CODEN: JOIMA C2 - 14707074 -- Source: Scopus

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