T cell functions in granulocyte/macrophage colony-stimulating factor deficient mice Journal Article
| Authors: | Wada, H.; Noguchi, Y.; Marino, M. W.; Dunn, A. R.; Old, L. J. |
| Article Title: | T cell functions in granulocyte/macrophage colony-stimulating factor deficient mice |
| Abstract: | Immunological functions were analyzed in mice lacking granulocyte/macrophage colony-stimulating factor (GM-CSF). The response of splenic T cells to alloantigens, anti-mouse CD3 mAb, interleukin 2 (IL-2), or concanavalin A was comparable in GM-CSF +/+ and GM-CSF -/- mice. To investigate the responses of CD8+ and CD4+ T cells against exogenous antigens, mice were immunized with ovalbumin peptide or with keyhole limpet hemocyanin (KLH). Cytotoxic CD8+ T cells with specificity for ovalbumin peptide could not be induced in GM-CSF -/- mice. After immunization with KLH, there was a delay in IgG generation, particularly IgG2a, in GM-CSF -/- mice. Purified CD4+ T cells from GM-CSF -/- mice immunized with KLH showed impaired proliferative responses and produced low amounts of interferon-γ (IFN-γ) and IL-4 when KLH-pulsed B cells or spleen cells were used as antigen presenting cells (APC). When enriched dendritic cells (DC) were used as APC, CD4+ T cells from GM-CSF -/- mice proliferated as well as those from GM-CSF +/+ mice and produced high amounts of IFN-γ and IL-4. To analyze the rescue effect of DC on CD4+ T cells, supernatants from (i) CD4+ T cells cultured with KLH-pulsed DC or (ii) DC cultured with recombinant GM-CSF were transferred to cultures of CD4+ T cells and KLH-pulsed spleen cells from GM- CSF -/- mice. Supernatants from both DC sources contained a factor or factors that restored proliferative responses and IFN-γ production of CD4+ T cells from GM-CSF -/- mice. |
| Keywords: | nonhuman; cd8 antigen; t lymphocyte; t-lymphocytes; animal cell; mouse; animals; mice; mice, knockout; interleukin 2; granulocyte macrophage colony stimulating factor; cytotoxicity; animalia; immunoglobulin g; cell culture; immunogenicity; immunity; t-lymphocyte subsets; cd4 antigen; antigen presenting cell; lymphocyte function; spleen cell; immunization; immunity, cellular; granulocyte-macrophage colony-stimulating factor; ovalbumin; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 94 |
| Issue: | 23 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1997-11-11 |
| Start Page: | 12557 |
| End Page: | 12561 |
| Language: | English |
| DOI: | 10.1073/pnas.94.23.12557 |
| PUBMED: | 9356488 |
| PROVIDER: | scopus |
| PMCID: | PMC25037 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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