Augmentation of antitumor immunity by tumor cells transduced with a retroviral vector carrying the interleukin-2 and interferon-γ cDNAs Journal Article

  

Authors:	Rosenthal, F. M.; Cronin, K.; Bannerji, R.; Golde, D. W.; Gansbacher, B.
Article Title:	Augmentation of antitumor immunity by tumor cells transduced with a retroviral vector carrying the interleukin-2 and interferon-γ cDNAs
Abstract:	Therapeutic models using gene transfer into tumor cells have pursued three objectives: (1) to induce rejection of the tumor transduced with therapeutic genes, (2) to induce immune-mediated regression of metastatic disease, and (3) to induce long-lasting immunity to protect against challenge with tumor cells or clinical regrowth of micrometastatic disease. Because in vivo therapy for patients with cancer using gene transfer would, as a first step, attempt to eliminate the existing tumor, we have investigated whether antitumor immunity induced by tumor cells secreting a single cytokine could be increased by cotransfer of a second cytokine gene. To test this approach, CMS-5, a murine fibrosarcoma, was transduced with retroviral vectors carrying interleukin-2 (IL-2), interferon-γ (IFN-γ), or granulocyte-macrophage- colony-stimulating factor (GM-CSF) cDNA alone or IL-2 cDNA in combination with IFN-γ or GM-CSF cDNA. Single cytokine-secreting clones were selected to match levels of cytokine production by double cytokine-secreting clones so that similar amounts of cytokine were secreted. IFN-γ- and IL-2/IFN-γ- secreting CMS-5 cells showed increased levels of major histocompatibility complex class I expression compared with IL-2- and GM-CSF-secreting or parental CMS-5 cells. IL-2/IFN-γ-secreting CMS-5 cells were always rejected by syngeneic mice, whereas the same number of CMS-5 cells secreting only one of these cytokines or mixtures of single cytokine-secreting CMS-5 cells were not rejected. In vivo depletion of CD4+, CD8+, or natural-killer effector cell subpopulations showed that CD8+ cytotoxic T cells were primarily responsible for rejection of IL-2/IFN-γ-transduced tumor cells. Our data show the successful use of a single retroviral vector to stably transduce two cytokine genes into the same tumor cell, leading to an increased effect on the in vivo induction of antitumor immunity.
Keywords:	nonhuman; flow cytometry; antigen expression; animal cell; mouse; animal; mice; interleukin 2; antineoplastic activity; tumor regression; transfection; mice, inbred balb c; gene transfer; genetic transduction; genetic vectors; fibrosarcoma; immunotherapy; gamma interferon; tumor cell; gene therapy; natural killer cell; immunity; graft rejection; neoplasm transplantation; major histocompatibility complex; complementary dna; retrovirus; retroviridae; interleukin-2; in vitro; major histocompatibility antigen class 1; sarcoma, experimental; interferon type ii; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title:	Blood
Volume:	83
Issue:	5
ISSN:	0006-4971
Publisher:	American Society of Hematology
Date Published:	1994-03-01
Start Page:	1289
End Page:	1298
Language:	English
PROVIDER:	scopus
PUBMED:	8118032
DOI:	10.1182/blood.V83.5.1289.1289
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028153727&partnerID=40&md5=7b37ffa3b79663de73b2db65c91611b0
Notes:	Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/137654