The role of IL-2 secreted from genetically modified tumor cells in the establishment of antitumor immunity Journal Article
| Authors: | Bannerji, R.; Arroyo, C. D.; Cordon-Cardo, C.; Gilboa, E. |
| Article Title: | The role of IL-2 secreted from genetically modified tumor cells in the establishment of antitumor immunity |
| Abstract: | Although the importance of CD4+ T cells for the induction of an effective CD8+ cytolytic T cell response is well documented, the mechanism by which MHC class II-negative tumor cells recruit CD4+ T help is not well understood. We have previously shown that IL-2 or IFN-γ gene-modified CMS-5 tumor cells do not grow in syngeneic mice; however, mice which rejected the cytokine-secreting tumor cells develop a protective immune response against a challenge with parental tumor cells. Here we show that rejection of IL-2- secreting CMS-5 cells is not mediated by T cells. However, establishment of a protective immune response against CMS-5 tumor cells requires the presence of both CD4+ and CD8+ T cell subsets during the period of immunization with IL-2-secreting CMS-5 cells as well as during the effector phase. Extensive histologic analysis has failed to detect the presence of T cells at the site of immunization with either IL-2- or IFN-γ-secreting CMS-5 cells. The main infiltrate at the site of inoculation with IL-2-secreting CMS-5 cells consisted of NK cells that appeared to play a role in their rejection. The predominant infiltrate at the site of inoculation with IFN-γ-secreting CMS- 5 cells consisted of macrophages. These observations argue against a direct role for the intact tumor cell in presenting either T helper or CTL epitopes to the immune system, and support the view that specialized APC are responsible for the in vivo priming of a T cell response against MHC class I- restricted Ag. |
| Keywords: | nonhuman; mouse; animals; mice; animal tissue; interleukin 2; animal experiment; mice, inbred balb c; cellular immunity; mice, nude; cd4-positive t-lymphocytes; t-lymphocytes, cytotoxic; rat; neoplasms, experimental; natural killer cell; killer cells, natural; tumor immunity; graft rejection; neoplasm transplantation; antigen-presenting cells; helper cell; interleukin-2; immunization; cancer immunology; interferon type ii; immunohistology; humans; priority journal; article |
| Journal Title: | Journal of Immunology |
| Volume: | 152 |
| Issue: | 5 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1994-03-01 |
| Start Page: | 2324 |
| End Page: | 2332 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 7907636 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
