The histone chaperone TAF-I/SET/INHAT is required for transcription in vitro of chromatin templates Journal Article
| Authors: | Gamble, M. J.; Erdjument-Bromage, H.; Tempst, P.; Freedman, L. P.; Fisher, R. P. |
| Article Title: | The histone chaperone TAF-I/SET/INHAT is required for transcription in vitro of chromatin templates |
| Abstract: | To uncover factors required for transcription by RNA polymerase II on chromatin, we fractionated a mammalian cell nuclear extract. We identified the histone chaperone TAF-I (also known as INHAT [inhibitor of histone acetyltransferase]), which was previously proposed to repress transcription, as a potent activator of chromatin transcription responsive to the vitamin D 3 receptor or to Gal4-VP16. TAF-I associates with chromatin in vitro and can substitute for the related protein NAP-1 in assembling chromatin onto cloned DNA templates in cooperation with the remodeling enzyme ATP-dependent chromatin assembly factor (ACF). The chromatin assembly and transcriptional activation functions are distinct, however, and can be dissociated temporally. Efficient transcription of chromatin assembled with TAF-I still requires the presence of TAF-I during the polymerization reaction. Conversely, TAF-I cannot stimulate transcript elongation when added after the other factors necessary for assembly of a preinitiation complex on naked DNA. Thus, TAF-I is required to facilitate transcription at a step after chromatin assembly but before transcript elongation. |
| Keywords: | controlled study; unclassified drug; human cell; protein function; mammalia; chromosomal proteins, non-histone; transcription initiation; protein; transcription, genetic; hela cell; hela cells; transcription factors; gene expression regulation; transcription regulation; recombinant fusion proteins; histone; chromatin; transactivator protein; polymerization; cell fractionation; genes, reporter; trans-activators; adenosine triphosphate; rna polymerase ii; chromatin assembly and disassembly; chaperone; nucleosome; dna template; molecular chaperones; templates, genetic; colecalciferol receptor; naked dna; receptors, calcitriol; protein nap 1; protein taf 1; transactivator protein gal4 vp16 |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 25 |
| Issue: | 2 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2005-01-01 |
| Start Page: | 797 |
| End Page: | 807 |
| Language: | English |
| DOI: | 10.1128/mcb.25.2.797-807.2005 |
| PUBMED: | 15632079 |
| PROVIDER: | scopus |
| PMCID: | PMC543418 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 33" - "Export Date: 24 October 2012" - "CODEN: MCEBD" - "Source: Scopus" |
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