Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription Journal Article
| Authors: | Sutton, A.; Bucaria, J.; Osley, M. A.; Sternglanz, R. |
| Article Title: | Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription |
| Abstract: | Transcription of the four yeast histone gene pairs (HTA1-HTB1, HTA2-HTB2, HHT1-HHF1, and HHT2-HHF2) is repressed during G1, G2, and M. For all except HTA2-HTB2, this repression requires several trans-acting factors, including the products of the HIR genes, HIR1, HIR2, and HIR3. ASF1 is a highly conserved protein that has been implicated in transcriptional silencing and chromatin assembly. In this analysis, we show that HIR1 interacts with ASF1 in a two-hybrid analysis. Further, asf1 mutants, like hir mutants, are defective in repression of histone gene transcription during the cell cycle and in cells arrested in early S phase in response to hydroxyurea, asf1 and hir1 mutations also show very similar synergistic interactions with mutations in cac2, a subunit of the yeast chromatin assembly factor CAF-I. The results suggest that ASF1 and HIR1 function in the same pathway to create a repressive chromatin structure in the histone genes during the cell cycle. |
| Keywords: | controlled study; unclassified drug; gene deletion; mutation; dna-binding proteins; hydroxyurea; nonhuman; flow cytometry; mutant protein; phenotype; cell cycle proteins; chromosomal proteins, non-histone; cell cycle; cell cycle s phase; protein protein interaction; protein binding; gene product; transcription, genetic; time factors; luminescent proteins; rna; blotting, western; transcription regulation; recombinant fusion proteins; histone; chromatin; gene repression; green fluorescent proteins; plasmids; cell nucleus; gene silencing; yeast; saccharomyces cerevisiae proteins; protein subunit; chromosome deletion; two hybrid system; cell separation; histones; genetic conservation; fungal protein; chromatin structure; two-hybrid system techniques; fungal proteins; priority journal; article; trans acting factor; protein asf1 |
| Journal Title: | Genetics |
| Volume: | 158 |
| Issue: | 2 |
| ISSN: | 0016-6731 |
| Publisher: | Genetics Society of America |
| Date Published: | 2001-06-01 |
| Start Page: | 587 |
| End Page: | 596 |
| Language: | English |
| PUBMED: | 11404324 |
| PROVIDER: | scopus |
| PMCID: | PMC1461693 |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
