Gene expression profiling in single cells within tissue Journal Article
| Authors: | Capodieci, P.; Donovan, M.; Buchinsky, H.; Jeffers, Y.; Cordon-Cardo, C.; Gerald, W.; Edelson, J.; Shenoy, S. M.; Singer, R. H. |
| Article Title: | Gene expression profiling in single cells within tissue |
| Abstract: | We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures. |
| Keywords: | signal transduction; histopathology; polymerase chain reaction; protein localization; reproducibility; in situ hybridization, fluorescence; gene expression profiling; neoplasm proteins; tumor markers, biological; genetic transcription; tumor cells, cultured; phosphatidylinositol 3 kinase; prediction; prostate cancer; prostatic neoplasms; tissue section; standardization; rna; correlation analysis; fluorescence in situ hybridization; cell culture; tissue distribution; quantitative analysis; prostate tumor; tumor cell; cellular distribution; benign tumor; computer program; protein kinase; protein microarray; formaldehyde; rna extraction; tissue structure; paraffin; paraffin embedding |
| Journal Title: | Nature Methods |
| Volume: | 2 |
| Issue: | 9 |
| ISSN: | 1548-7091 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2005-09-01 |
| Start Page: | 663 |
| End Page: | 665 |
| Language: | English |
| DOI: | 10.1038/nmeth786 |
| PUBMED: | 16118636 |
| PROVIDER: | scopus |
| PMCID: | PMC1976602 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 26" - "Export Date: 24 October 2012" - "Source: Scopus" |
