Tetracycline-mediated suppression of gene expression with a new dicistronic retroviral vector Journal Article
| Authors: | Kim, S. J.; Sadelain, M.; Choi, K. H.; Kim, H. K.; Lee, J. S.; Chung, H. Y. |
| Article Title: | Tetracycline-mediated suppression of gene expression with a new dicistronic retroviral vector |
| Abstract: | Since the first description of the helper-free retrovirus vector by Mann et al. (1983), many improvements have been introduced to the system to increase titer, or to achieve better expression of the transduced genes in cells of different lineage. The typical form of recombinant retrovirus vector utilizes its 5′ long terminal repeat (LTR) as a promoter unit for the transcription of the inserted gene(s), which allows only the constitutive expression of the inserted gene(s) in target cells. Although constitutive expression of the delivered gene(s) in target cells may be sufficient for some purposes, controlled expression of gene(s) in target cells or tissues would be favorable in many basic and clinical applications. To circumvent these problems, we developed a new retroviral vector that allows controlled expression of the inserted genes. With these new retroviral vectors, the expression level of the inserted genes can be controlled up to 200-fold in the presence of a minimum amount of tetracycline. We think these new retroviral vectors will be useful in regulating the expression of the genes delivered in vivo. |
| Keywords: | genetics; mouse; animal; metabolism; animals; mice; granulocyte macrophage colony stimulating factor; drug effect; tumor cells, cultured; transfection; physiology; gene vector; genetic vectors; gene expression regulation; biosynthesis; genetic transfection; cell culture; leukemia, myeloid; virus replication; tetracycline; cell strain 3t3; synthesis; retrovirus; retroviridae; 3t3 cells; virus protein; viral structural proteins; myeloid leukemia; virus gene; granulocyte-macrophage colony-stimulating factor; virion; genes, viral; article; encephalomyocarditis virus |
| Journal Title: | Molecules and Cells |
| Volume: | 7 |
| Issue: | 4 |
| ISSN: | 1016-8478 |
| Publisher: | Ksmcb 2008 |
| Date Published: | 1997-08-01 |
| Start Page: | 514 |
| End Page: | 520 |
| Language: | English |
| PUBMED: | 9339896 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
