Retroviral-mediated gene transfer in primary murine and human T-lymphocytes Journal Article
| Authors: | Riviere, I.; Gallardo, H. F.; Hagani, A. B.; Sadelain, M. |
| Article Title: | Retroviral-mediated gene transfer in primary murine and human T-lymphocytes |
| Abstract: | Recombinant retroviruses are efficient vectors for introducing genes into many mammalian cell types. They are useful in the context of clinical as well as experimental applications, owing to the ability to generate high-titer and helper-free viral stocks. Retroviral vectors are especially appropriate for the transduction of primary lymphocytes, because gene transfer is stable and mediated by nonimmunogenic vectors. Stable integration in chromosomes of cells undergoing clonal expansion ensures that the foreign genetic material will be faithfully transmitted to the cells' progeny. However, oncoretroviral vectors derived from murine leukemia viruses (MLV) require target cell division to integrate. Here we review factors that determine retroviral-mediated gene transfer efficiency in primary T-lymphocytes, in particular. T-cell activation status, viral receptor expression, and culture conditions. |
| Keywords: | human cell; nonhuman; flow cytometry; t lymphocyte; t-lymphocytes; animal cell; mammalia; animals; cell survival; cells, cultured; cell division; gene expression; membrane proteins; transfection; virus receptor; gene transfer; genetic transduction; animalia; genetic vectors; receptors, virus; lymphocyte activation; carrier proteins; gene therapy; murinae; virus recombinant; lymphocyte; t lymphocyte activation; retrovirus; retroviridae; expression vector; transduction; retroviral vector; dna integration; murine leukemia virus; leukemia virus, murine; unidentified retrovirus; humans; human; article; amino acid transport systems, basic; phosphate-binding proteins |
| Journal Title: | Applied Biochemistry and Biotechnology |
| Volume: | 15 |
| Issue: | 2 |
| ISSN: | 1073-6085 |
| Publisher: | Humana Press Inc |
| Date Published: | 2000-06-01 |
| Start Page: | 133 |
| End Page: | 142 |
| Language: | English |
| PUBMED: | 10949826 |
| PROVIDER: | scopus |
| DOI: | 10.1385/MB:15:2:133 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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