Molecular profiling of tumor progression in head and neck cancer Journal Article

  


Authors: Belbin, T. J.; Singh, B.; Smith, R. V.; Socci, N. D.; Wreesmann, V. B.; Sanchez Carbayo, M.; Masterson, J.; Patel, S.; Cordon-Cardo, C.; Prystowsky, M. B.; Childs, G.
Article Title: Molecular profiling of tumor progression in head and neck cancer
Abstract: Objective: To assess gene expression changes associated with tumor progression in patients with squamous cell carcinoma of the oral cavity. Design: A microarray containing 17840 complementary DNA clones was used to measure gene expression changes associated with tumor progression in 9 patients with squamous cell carcinoma of the oral cavity. Samples were taken for analysis from the primary tumor, nodal metastasis, and "normal" mucosa from the patients' oral cavity. Setting: Tertiary care facility. Patients: Nine patients with stage III or stage IV untreated oral cavity squamous cell carcinoma. Results: Our analysis to categorize genes based on their expression patterns has identified 140 genes that consistently increased in expression during progression from normal tissue to invasive tumor and subsequently to metastatic node (in at least 4 of the 9 cases studied). A similar list of 94 genes has been identified that decreased in expression during tumor progression and metastasis. We validated this gene discovery approach by selecting moesin (a member of the ezrin/radixin/ moesin [ERM] family of cytoskeletal proteins) and one of the genes that consistently increased in expression during tumor progression for subsequent immunohistochemical analysis using a head and neck squamous cell carcinoma tissue array. Conclusion: A distinct pattern of gene expression, with progressive up- or down-regulation of expression, is found during the progression from histologically normal tissue to primary carcinoma and to nodal metastasis.
Keywords: immunohistochemistry; clinical article; controlled study; human tissue; squamous cell carcinoma; carcinoma, squamous cell; neoplasm staging; genetic analysis; metastasis; gene expression; gene expression profiling; microfilament proteins; molecular cloning; dna; head and neck neoplasms; oligonucleotide array sequence analysis; disease progression; gene identification; nucleotide sequence; dna fingerprinting; dna microarray; tumor growth; tumor; unindexed sequence; mouth carcinoma; mouth; complementary dna; moesin; neck tumor; head tumor
Journal Title: Archives of Otolaryngology - Head & Neck Surgery
Volume: 131
Issue: 1
ISSN: 0886-4470
Publisher: American Medical Association  
Date Published: 2005-01-01
Start Page: 10
End Page: 18
Language: English
DOI: 10.1001/archotol.131.1.10
PUBMED: 15655179
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-19944428798&partnerID=40&md5=69d3770a6c4e49174384b3ebcf93a056
Notes: --- - "Cited By (since 1996): 41" - "Export Date: 24 October 2012" - "CODEN: AONSE" - "Molecular Sequence Numbers: GENBANK: AA011215, AA017544, AA085318, AA086475, AA132090, AA278759, AA284954, AA291163, AA292074, AA425224, AA446994, AA456474, AA459380, AA464578, AA475240, AA478585, AA485371, AA487031, AA495724, AA630374, AA644088, AA664004, AA677534, AA701652, AA775616, AA857212, H06113, H29295, H38240, H45976, H48501, H51574, H61935, H70373, H91826, N64384, N66144, R22977, R33031, R43956, R62612, R69307, R71440, R78530, R83836, T69304, T77595, T95052, W21482, W81196;" - "Source: Scopus"
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MSK Authors
  1. Bhuvanesh Singh
    242 Singh
  2. Snehal G Patel
    412 Patel
  3. Volkert B Wreesmann
    22 Wreesmann
  4. Carlos Cordon-Cardo
    612 Cordon-Cardo
  5. Marta Sanchez-Carbayo Martin
    38 Sanchez-Carbayo Martin
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