Familial transmission rather than defective innate immunity shapes the distinct intestinal microbiota of TLR-deficient mice Journal Article
| Authors: | Ubeda, C.; Lipuma, L.; Gobourne, A.; Viale, A.; Leiner, I.; Equinda, Michele; Khanin, R.; Pamer, E. G. |
| Article Title: | Familial transmission rather than defective innate immunity shapes the distinct intestinal microbiota of TLR-deficient mice |
| Abstract: | The intestinal microbiota contributes to the development of the immune system, and conversely, the immune system influences the composition of the microbiota. Toll-like receptors (TLRs) in the gut recognize bacterial ligands. Although TLR signaling represents a major arm of the innate immune system, the extent to which TLRs influence the composition of the intestinal microbiota remains unclear. We performed deep 16S ribosomal RNA sequencing to characterize the complex bacterial populations inhabiting the ileum and cecum of TLR- and MyD88-deficient mice. The microbiota of MyD88- and TLR-deficient mouse colonies differed markedly, with each colony harboring distinct and distinguishable bacterial populations in the small and large intestine. Comparison of MyD88-, TLR2-, TLR4-, TLR5-, and TLR9-deficient mice and their respective wild-type (WT) littermates demonstrated that the impact of TLR deficiency on the composition of the intestinal microbiota is minimal under homeostatic conditions and after recovery from antibiotic treatment. Thus, differences between TLR-deficient mouse colonies reflected long-term divergence of the microbiota after extended husbandry in isolation from each other. Longterm breeding of isolated mouse colonies results in changes of the intestinal microbiota that are communicated to offspring by maternal transmission, which account for marked compositional differences between WT and mutant mouse strains. © 2012 Ubeda et al. |
| Keywords: | signal transduction; controlled study; antibiotic therapy; sequence analysis; nonhuman; mouse; animals; mice; mice, knockout; gene amplification; animal experiment; wild type; mice, inbred c57bl; anti-bacterial agents; progeny; innate immunity; immunity, innate; vancomycin; intestine flora; homeostasis; rna 16s; myeloid differentiation factor 88; toll like receptor; cecum; ileum; family; disease transmission; bacterium isolation; toll-like receptors; bacterium mutant; breeding; metagenome; bacterium colony; rna, ribosomal, 16s |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 209 |
| Issue: | 8 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2012-07-30 |
| Start Page: | 1445 |
| End Page: | 1456 |
| Language: | English |
| DOI: | 10.1084/jem.20120504 |
| PROVIDER: | scopus |
| PMCID: | PMC3409501 |
| PUBMED: | 22826298 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 October 2012" - "CODEN: JEMEA" - "Source: Scopus" |
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