MyD88-mediated signals induce the bactericidal lectin RegIIIγ and protect mice against intestinal Listeria monocytogenes infection Journal Article

   

Authors:	Brandl, K.; Plitas, G.; Schnabl, B.; DeMatteo, R. P.; Pamer, E. G.
Article Title:	MyD88-mediated signals induce the bactericidal lectin RegIIIγ and protect mice against intestinal Listeria monocytogenes infection
Abstract:	Listeria monocytogenes is a food-borne bacterial pathogen that causes systemic infection by traversing the intestinal mucosa. Although MyD88-mediated signals are essential for defense against systemic L. monocytogenes infection, the role of Toll-like receptor and MyD88 signaling in intestinal immunity against this pathogen has not been defined. We show that clearance of L. monocytogenes from the lumen of the distal small intestine is impaired in MyD88-/- mice. The distal ileum of wild-type (wt) mice expresses high levels of RegIIIγ, which is a bactericidal lectin that is secreted into the bowel lumen, whereas RegIIIγ expression in MyD88-/- mice is nearly undetectable. In vivo depletion of RegIIIγ from the small intestine of wt mice diminishes killing of luminal L. monocytogenes, whereas reconstitution of MyD88-deficient mice with recombinant RegIIIγ enhances intestinal bacterial clearance. Experiments with bone marrow chimeric mice reveal that MyD88-mediated signals in nonhematopoietic cells induce RegIIIγ expression in the small intestine, thereby enhancing bacterial killing. Our findings support a model of MyD88-mediated epithelial conditioning that protects the intestinal mucosa against bacterial invasion by inducing RegIIIγ. JEM © The Rockefeller University Press.
Keywords:	controlled study; protein expression; unclassified drug; nonhuman; proteins; mouse; animals; mice; animal tissue; bone marrow cells; animal experiment; animal model; protein; in vivo study; wild type; mice, inbred c57bl; mice, transgenic; gene expression regulation; kinetics; recombinant proteins; hematopoietic cell; immunity; disease predisposition; small intestine; intestines; myeloid differentiation factor 88; listeria monocytogenes; listeria infections; intestine mucosa; toll-like receptors; lectin; bactericidal activity; regiiigamma; lectins
Journal Title:	Journal of Experimental Medicine
Volume:	204
Issue:	8
ISSN:	0022-1007
Publisher:	Rockefeller University Press
Date Published:	2007-08-06
Start Page:	1891
End Page:	1900
Language:	English
DOI:	10.1084/jem.20070563
PUBMED:	17635956
PROVIDER:	scopus
PMCID:	PMC2118673
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-34547762705&partnerID=40&md5=f001037d81e62b273301c3aa122bb400
Notes:	--- - "Cited By (since 1996): 68" - "Export Date: 17 November 2011" - "CODEN: JEMEA" - "Source: Scopus"

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