An atlas of the Epstein-Barr virus transcriptome and epigenome reveals host-virus regulatory interactions Journal Article

   

Authors:	Arvey, A.; Tempera, I.; Tsai, K.; Chen, H. S.; Tikhmyanova, N.; Klichinsky, M.; Leslie, C.; Lieberman, P. M.
Article Title:	An atlas of the Epstein-Barr virus transcriptome and epigenome reveals host-virus regulatory interactions
Abstract:	Epstein-Barr virus (EBV), which is associated with multiple human tumors, persists as a minichromosome in the nucleus of B lymphocytes and induces malignancies through incompletely understood mechanisms. Here, we present a large-scale functional genomic analysis of EBV. Our experimentally generated nucleosome positioning maps and viral protein binding data were integrated with over 700 publicly available high-throughput sequencing data sets for human lymphoblastoid cell lines mapped to the EBV genome. We found that viral lytic genes are coexpressed with cellular cancer-associated pathways, suggesting that the lytic cycle may play an unexpected role in virus-mediated oncogenesis. Host regulators of viral oncogene expression and chromosome structure were identified and validated, revealing a role for the B cell-specific protein Pax5 in viral gene regulation and the cohesin complex in regulating higher order chromatin structure. Our findings provide a deeper understanding of latent viral persistence in oncogenesis and establish a valuable viral genomics resource for future exploration. © 2012 Elsevier Inc.
Keywords:	gene sequence; nonhuman; chromosome structure; gene expression; epigenetics; gene control; virus genome; transcriptome; epstein barr virus; herpesvirus 4, human; virus protein; viral proteins; epstein-barr virus infections; gene expression regulation, viral; virus oncogene; host-pathogen interactions; virus cell interaction; epigenomics; transcription factor pax5; b-cell-specific activator protein
Journal Title:	Cell Host & Microbe
Volume:	12
Issue:	2
ISSN:	1931-3128
Publisher:	Cell Press
Date Published:	2012-08-16
Start Page:	233
End Page:	245
Language:	English
DOI:	10.1016/j.chom.2012.06.008
PROVIDER:	scopus
PMCID:	PMC3424516
PUBMED:	22901543
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84865137988&partnerID=40&md5=417e466630d227c5bfaafe7249d38e71
Notes:	--- - "Export Date: 4 September 2012" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/33253