Polypodium leucotomos decreases UV-induced epidermal cell proliferation and enhances p53 expression and plasma antioxidant capacity in hairless mice Journal Article
| Authors: | Rodríguez-yanes, E.; Juarranz, A.; Cuevas, J.; Gonzalez, S.; Mallol, J. |
| Article Title: | Polypodium leucotomos decreases UV-induced epidermal cell proliferation and enhances p53 expression and plasma antioxidant capacity in hairless mice |
| Abstract: | A single dose of ultraviolet radiation (UVR) induces significant changes in blood and skin of hairless mice. Oral administration of a hydrophilic extract of the fern Polypodium leucotomos (PL, 300 mg/kg during 5 days before UVR and for two additional days after irradiation) modulates some of the effects of UVR. Most significantly, PL administration reduced the number of proliferating cells by 13%, increased the number of p53 + cells by 63%, enhanced the antioxidant plasma capacity (ORAC) by 30% and reinforced the network of dermal elastic fibres. Western blot analysis of skin antioxidant-related enzymes failed to demonstrate significant changes caused by PL. Thus, the beneficial effect of PL likely owes to its antioxidant and anti-ROS properties rather than its modulation of the expression of endogenous antioxidant systems. These data provide mechanistic clues for its efficacy as a systemic photoprotective agent with antioxidant and anti-photo-ageing properties. © 2012 John Wiley & Sons A/S. |
| Keywords: | protein expression; nonhuman; letter; ultraviolet radiation; cell proliferation; mouse; animals; mice; animal tissue; epidermis; animal experiment; protein p53; dose-response relationship, radiation; models, animal; western blotting; irradiation; tumor suppressor protein p53; single drug dose; plant extracts; antioxidant; antioxidants; antioxidant activity; ultraviolet rays; administration, oral; superoxide dismutase; p53; polypodium leucotomos extract; polypodium; skin aging; hairless mouse; mice, hairless; photoprotection; polypodium leucotomos; elastic tissue; epidermis cell; natural plant extract |
| Journal Title: | Experimental Dermatology |
| Volume: | 21 |
| Issue: | 8 |
| ISSN: | 0906-6705 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2012-08-01 |
| Start Page: | 638 |
| End Page: | 640 |
| Language: | English |
| DOI: | 10.1111/j.1600-0625.2012.01544.x |
| PROVIDER: | scopus |
| PUBMED: | 22776002 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 August 2012" - "CODEN: EXDEE" - "Source: Scopus" |
