Near instrument-free, simple molecular device for rapid detection of herpes simplex viruses Journal Article
| Authors: | Lemieux, B.; Li, Y.; Kong, H.; Tang, Y. W. |
| Article Title: | Near instrument-free, simple molecular device for rapid detection of herpes simplex viruses |
| Abstract: | The first near instrument-free, inexpensive and simple molecular diagnostic device (IsoAmp ® HSV, BioHelix Corp., MA, USA) recently received US FDA clearance for use in the detection of herpes simplex viruses (HSV) in genital and oral lesion specimens. The IsoAmp HSV assay uses isothermal helicase-dependent amplification in combination with a disposable, hermetically-sealed, vertical-flow strip identification. The IsoAmp HSV assay has a total test-to-result time of less than 1.5 h by omitting the time-consuming nucleic acid extraction. The diagnostic sensitivity and specificity are comparable to PCR and are superior to culture-based methods. The near instrument-free, rapid and simple characteristics of the IsoAmp HSV assay make it potentially suitable for point-of-care testing. © 2012 Expert Reviews Ltd. |
| Keywords: | review; nonhuman; sensitivity and specificity; dna polymerase; reproducibility; gene amplification; food and drug administration; cost effectiveness analysis; mycobacterium tuberculosis; cell culture; simplexvirus; dna, viral; human immunodeficiency virus; helicase; methicillin resistant staphylococcus aureus; clostridium difficile; virus strain; herpes simplex virus 1; herpes simplex virus; virus particle; virus detection; vagina smear; virus isolation; molecular diagnostic techniques; genital system disease; oral biopsy; sexually transmitted disease; herpes simplex virus 2; limit of detection; devices; point of care testing; nucleic acid amplification techniques; workflow; chlamydia trachomatis; streptococcus agalactiae; genital lesion; helicase-dependent amplification; isothermal nucleic acid amplification; point-of-care testing; vertical flow test strip; elvis hsv id; isoamp hsv; mouth lesion; multicode hsv; neisseria gonorrhoeae; probetec hsv; herpes genitalis; stomatitis, herpetic |
| Journal Title: | Expert Review of Molecular Diagnostics |
| Volume: | 12 |
| Issue: | 5 |
| ISSN: | 1473-7159 |
| Publisher: | Informa Healthcare |
| Date Published: | 2012-06-01 |
| Start Page: | 437 |
| End Page: | 443 |
| Language: | English |
| DOI: | 10.1586/erm.12.34 |
| PROVIDER: | scopus |
| PUBMED: | 22702360 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 July 2012" - "CODEN: ERMDC" - "Source: Scopus" |
