Evaluation of alere i influenza A&B for rapid detection of influenza viruses A and B Journal Article
| Authors: | Nie, S.; Roth, R. B.; Stiles, J.; Mikhlina, A.; Lu, X.; Tang, Y. W.; Babady, N. E. |
| Article Title: | Evaluation of alere i influenza A&B for rapid detection of influenza viruses A and B |
| Abstract: | Rapid and accurate diagnosis of influenza is important for infection control, as well as for patient management. Alere i Influenza A&B is an isothermal nucleic acid amplification-based integrated system for detection and differentiation of influenza virus A and influenza virus B. The performance of the Alere i Influenza A&B was screened using frozen nasopharyngeal-swab specimens collected in viral transport medium (VTM) that were originally tested fresh with the FilmArray Respiratory Panel (RP) assay during the 2012-2013 influenza outbreak. In total, 360 VTM specimens were selected for Alere i Influenza A&B testing: 40 influenza virus A H1N1-2009 (influenza virus A-1), 40 influenza virus A H3N2 (influenza virus A-3), 37 influenza virus A "equivocal" or "no subtype detected" (influenza virus A-u), 41 influenza virus B, and 202 influenza virus-negative specimens, as initially determined by the FilmArray RP assay. The Alere assay showed sensitivities of 87.2%, 92.5%, 25.0%, and 97.4% for influenza virus A-1, influenza virus A-3, influenza virus A-u, and influenza virus B, respectively, after discordant resolution by Prodesse Pro- FLU + PCR. The specificities were 100% for both influenza virus A and influenza virus B. In general, the Alere i Influenza A&B provided good sensitivity, although the assay did show poorer sensitivity with samples determined to have low influenza virus A titers by Prodesse ProFlu+ PCR (a mean real-time PCR threshold cycle [CT] value of 31.9 ± 2.0), which included the majority of the samples called influenza virus A "equivocal" or "no subtype detected" by a single BioFire FilmArray RP test. The integrated, rapid, and simple characteristics of the Alere i Influenza A&B assay make it a potential candidate for point-of-care testing, with a test turnaround time of less than 15 min. Copyright © 2014, American Society for Microbiology. All Rights Reserved. |
| Journal Title: | Journal of Clinical Microbiology |
| Volume: | 52 |
| Issue: | 9 |
| ISSN: | 0095-1137 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2014-09-01 |
| Start Page: | 3339 |
| End Page: | 3344 |
| Language: | English |
| DOI: | 10.1128/jcm.01132-14 |
| PROVIDER: | scopus |
| PUBMED: | 24989611 |
| PMCID: | PMC4313160 |
| DOI/URL: | |
| Notes: | Export Date: 1 October 2014 -- Source: Scopus |
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