Reconstitution, activities, and structure of the eukaryotic RNA exosome Journal Article


Authors: Liu, Q.; Greimann, J. C.; Lima, C. D.
Article Title: Reconstitution, activities, and structure of the eukaryotic RNA exosome
Abstract: The RNA exosome is a multisubunit 3′ to 5′ exoribonuclease complex that participates in degradation and processing of cellular RNA. To determine the activities and structure of the eukaryotic exosome, we report the reconstitution of 9-subunit exosomes from yeast and human and reconstitution of 10- and 11-subunit exosomes from yeast. Comparative biochemical analysis between purified subunits and reconstituted exosomes using AU-rich, polyadenylated (poly[A]), generic, and structured RNA substrates reveals processive phosphorolytic activities for human Rrp41/Rrp45 and the 9-subunit human exosome, processive hydrolytic activities for yeast Rrp44 and the yeast 10-subunit exosome, distributive hydrolytic activities for Rrp6, and processive and distributive hydrolytic activities for the yeast 11-subunit exosome. To elucidate the architecture of a eukaryotic exosome, its conserved surfaces, and the structural basis for RNA decay, we report the X-ray structure determination for the 286 kDa nine-subunit human exosome at 3.35 Å. © 2006 Elsevier Inc. All rights reserved.
Keywords: controlled study; protein phosphorylation; human cell; nonhuman; animal cell; animal tissue; structure activity relation; structure-activity relationship; rna; protein processing; amino acid sequence; molecular sequence data; saccharomyces cerevisiae; sequence alignment; eukaryota; substrate specificity; pregnancy; models, molecular; crystallography, x-ray; mutagenesis, site-directed; protein structure, tertiary; rna stability; saccharomyces cerevisiae proteins; rna structure; rna processing; hydrolysis; placenta; archaeal proteins; dna, complementary; adenylation; rna degradation; rna analysis; exosome; exoribonucleases
Journal Title: Cell
Volume: 127
Issue: 6
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 2006-12-15
Start Page: 1223
End Page: 1237
Language: English
DOI: 10.1016/j.cell.2006.10.037
PUBMED: 17174896
PROVIDER: scopus
DOI/URL:
Notes: --- - "Cited By (since 1996): 166" - "Export Date: 4 June 2012" - "CODEN: CELLB" - "Source: Scopus"
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MSK Authors
  1. Quansheng Liu
    3 Liu
  2. Christopher D Lima
    95 Lima