Reconstitution, activities, and structure of the eukaryotic RNA exosome Journal Article
| Authors: | Liu, Q.; Greimann, J. C.; Lima, C. D. |
| Article Title: | Reconstitution, activities, and structure of the eukaryotic RNA exosome |
| Abstract: | The RNA exosome is a multisubunit 3′ to 5′ exoribonuclease complex that participates in degradation and processing of cellular RNA. To determine the activities and structure of the eukaryotic exosome, we report the reconstitution of 9-subunit exosomes from yeast and human and reconstitution of 10- and 11-subunit exosomes from yeast. Comparative biochemical analysis between purified subunits and reconstituted exosomes using AU-rich, polyadenylated (poly[A]), generic, and structured RNA substrates reveals processive phosphorolytic activities for human Rrp41/Rrp45 and the 9-subunit human exosome, processive hydrolytic activities for yeast Rrp44 and the yeast 10-subunit exosome, distributive hydrolytic activities for Rrp6, and processive and distributive hydrolytic activities for the yeast 11-subunit exosome. To elucidate the architecture of a eukaryotic exosome, its conserved surfaces, and the structural basis for RNA decay, we report the X-ray structure determination for the 286 kDa nine-subunit human exosome at 3.35 Å. © 2006 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; protein phosphorylation; human cell; nonhuman; animal cell; animal tissue; structure activity relation; structure-activity relationship; rna; protein processing; amino acid sequence; molecular sequence data; saccharomyces cerevisiae; sequence alignment; eukaryota; substrate specificity; pregnancy; models, molecular; crystallography, x-ray; mutagenesis, site-directed; protein structure, tertiary; rna stability; saccharomyces cerevisiae proteins; rna structure; rna processing; hydrolysis; placenta; archaeal proteins; dna, complementary; adenylation; rna degradation; rna analysis; exosome; exoribonucleases |
| Journal Title: | Cell |
| Volume: | 127 |
| Issue: | 6 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2006-12-15 |
| Start Page: | 1223 |
| End Page: | 1237 |
| Language: | English |
| DOI: | 10.1016/j.cell.2006.10.037 |
| PUBMED: | 17174896 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 166" - "Export Date: 4 June 2012" - "CODEN: CELLB" - "Source: Scopus" |
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